| 玉米Mn-SOD的分离纯化与性质分析 |
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| 引用本文： | 赵晓瑜,宋小青,刘建荣.玉米Mn-SOD的分离纯化与性质分析[J].河北大学学报(自然科学版),2009,29(3):324. |
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| 作者姓名： | 赵晓瑜 宋小青 刘建荣 |
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| 作者单位： | 1.河北大学,生命科学学院,河北,保定071002； 2.河北北方学院,医学院,河北,张家口075000 |
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| 摘 要： | 利用金属螯合亲和层析,结合DEAE-纤维素离子交换层析,从玉米匀浆液中分离纯化到电泳纯的Mn-SOD组分.双向电泳和SDS-PAGE分析表明:玉米Mn-SOD的亚基分子质量为26.2 kD,等电点为5.3;热稳定性、酸碱稳定性高于玉米Cu/Zn-SOD.
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| 关 键 词： | 玉米Mn-SOD 金属螯合亲和层析 纯化 |
Purification and Analysis of Mn-SOD from Maize |
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| ZHAO Xiao-yu,SONG Xiao-qing,LIU Jian-rong.Purification and Analysis of Mn-SOD from Maize[J].Journal of Hebei University (Natural Science Edition),2009,29(3):324. |
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| Authors: | ZHAO Xiao-yu SONG Xiao-qing LIU Jian-rong |
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| Abstract: | Metal chelate affinity chromatography and DEAE-cellulose ion-exchange chromatography were used to separate and purify SOD from maize in this research.A electrophoretic pure Mn-SOD component was obtained.The SDS-PAGE and 2-D electrophoresis showed that the subunit of the Mn-SOD was 26.2 kD and its isoelectric point was 5.3.The experimentation of stability showed that the Mn-SOD component had higher thermal stability and pH stability than Cu/Zn-SOD. |
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| Keywords: | maize Mn-SOD metal chelate affinity chromatography purification |
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