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| 氨氮对斑马鱼2种代谢酶类的影响 | |
| 引用本文： | 韩力强,康现江,李双石,温秀荣,李云峰,李凤超.氨氮对斑马鱼2种代谢酶类的影响[J].河北大学学报(自然科学版),2005,25(2):179-184. |
| 作者姓名： | 韩力强 康现江 李双石 温秀荣 李云峰 李凤超 |
| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002;河北省渔政处,河北,秦皇岛,066001 2. 河北大学,生命科学学院,河北,保定,071002 3. 北京电子科技职业学院,工程技术系,北京,100029 |
| 基金项目： | 河北省自然科学基金资助项目(303118) |
| 摘 要： | 以斑马鱼(Brachyclaniorerio)为实验材料,用氯化铵模拟水体中氨氮进行急性毒性实验.得到24,48,72,96h的半致死浓度(LC50),分别为126,114,105,101mg/L.在96hLC50以下,设6个质量浓度梯度,进行24h氨氮胁迫,之后测定斑马鱼不同组织中的过氧化氢酶(CATase),腺三磷酸酶(ATPase)活性.结果表明,各类酶活的变化与氨氮质量浓度都呈一定相关性,随着氨氮质量浓度增大,ATP酶活性受到显著抑制,CAT酶呈曲线变化. |
| 关 键 词： | 斑马鱼 氨氮胁迫 CAT ATP |
| 文章编号： | 1000-1565(2005)02-0179-06 |
| 修稿时间： | 2004年8月10日 |
Effects of Ammonia Nitrogen on Metabolic Enzymes in Brachyclanio rerio |
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| HAN Li-qiang,KANG Xian-jiang,LI Shuang-shi,WEN Xiu-rong,LI Yun-feng,LI Feng-chao.Effects of Ammonia Nitrogen on Metabolic Enzymes in Brachyclanio rerio[J].Journal of Hebei University (Natural Science Edition),2005,25(2):179-184. | |
| Authors: | HAN Li-qiang KANG Xian-jiang LI Shuang-shi WEN Xiu-rong LI Yun-feng LI Feng-chao |
| Institution: | HAN Li-qiang~ |
| Abstract: | Using chlorine ammonium to simulate ammonia nitrogen in water , the acute toxicity test was carried out to Brachyclanio rerio. The half lethal concentration (LC_(50)) values of 24, 48, 72 and 96 h were 126, 114, 105, 101 mg/L respectively. Then six gradient treatments were set up under the 96 h LC_(50). According to the treatments, after exposure to 24 h with ammonia nitrogen stress, the activities of Catalase (CAT) , Adenosine triphosphatase (ATPase) in zebrafish's different tissues were measured. The results showed that, the changes of all enzymes activities were closely related to the concentrations of ammonia nitrogen; along with the increasing concentrations of ammonia nitrogen, ATPase activity was suppressed notably; CATase presented curvilinear motion. |
| Keywords: | Brachyclanio rerio ammonia nitrogen stress CAT ATP |
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