| 碱裂解提取质粒DNA的改进方法 |
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| 引用本文： | 倪志华,赵晓瑜,刘建荣,王三元,毕智丽,周欣.碱裂解提取质粒DNA的改进方法[J].河北大学学报(自然科学版),2005,25(2):222-224. |
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| 作者姓名： | 倪志华 赵晓瑜 刘建荣 王三元 毕智丽 周欣 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省教育厅资助项目(2003204) |
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| 摘 要： | 实验室中常用的质粒提取方法是碱裂解法.一般碱裂解法提取质粒相对耗时长,而且需要冰浴,条件较为严格,本文尝试对碱裂解法进行改进,即在控制菌体量的前提下连续加入溶液Ⅰ,Ⅱ,Ⅲ,进行质粒提取,简化了操作步骤,缩短了实验时间(由150min左右缩短为110min以内),质粒收率和质量不变.
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| 关 键 词： | 质粒 碱裂解法 改进法 |
| 文章编号： | 1000-1565(2005)02-0222-03 |
| 修稿时间： | 2004年7月10日 |
A Modified Alkaline Lysis Method for Extracting Plasmid DNA |
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| NI Zhi-hua,ZHAO Xiao-yu,LIU Jian-rong,WANG San-yuan,BI Zhi-li,ZHOU Xin.A Modified Alkaline Lysis Method for Extracting Plasmid DNA[J].Journal of Hebei University (Natural Science Edition),2005,25(2):222-224. |
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| Authors: | NI Zhi-hua ZHAO Xiao-yu LIU Jian-rong WANG San-yuan BI Zhi-li ZHOU Xin |
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| Abstract: | The traditional alkaline method to isolate plasmid DNA takes time and the operation system requires strict control. The paper improved the method by putting solution Ⅰ,Ⅱ,Ⅲ one by one in the reaction system with the bacteria amount under control. The improved procedure, named a modified method, predigested steps and saved experimental time, while the quantity and the quality of plasmid DNA were maintained. |
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| Keywords: | plasmid DNA alkaline lysis method modified method |
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