| 靶向基因疫苗pcDNA3/MDC-VP1的构建及免疫效果 |
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| 引用本文： | 王晓凌,吴淑慧,高志云,王永祥.靶向基因疫苗pcDNA3/MDC-VP1的构建及免疫效果[J].河北大学学报(自然科学版),2006,26(1):16-20. |
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| 作者姓名： | 王晓凌 吴淑慧 高志云 王永祥 |
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| 作者单位： | 河北医科大学,病原生物学教研室,河北,石家庄,050017 |
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| 摘 要： | 用RT-PCR扩增小鼠巨噬细胞源趋化因子(macrophage-derived chemokine,MDC)基因,与柯萨奇病毒B组3型(CVB3)VP1基因通过一个编码10个氨基酸残基的接头序列(Gly4Ser)2相连,形成融合基因MDC-VP1,构建真核表达质粒pcDNA3/MDC-VP1,作为疫苗免疫BALB/c小鼠.3次免疫后,pcDNA3/MDC-VP1组小鼠血清中和抗体滴度明显高于pcDNA3/VP1组;而病毒滴度低于pcDNA3/VP1组.用10 LD50CVB3攻击,pcDNA3/MDC-VP1组小鼠生存率为50%,用Kaplan-Meier法进行生存分析,生存率高于其他各组.
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| 关 键 词： | 巨噬细胞源趋化因子 柯萨奇病毒B组3型 融合基因 中和抗体 小鼠 |
| 文章编号： | 1000-1565(2006)01-0016-05 |
| 修稿时间： | 2005年8月17日 |
Construction of Targeting DNA Vaccine pcDNA3/MDC-VP1 and its Immunological Effect on Mice |
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| WANG Xiao-ling,WU Shu-hui,GAO Zhi-yun,WANG Yong-xiang.Construction of Targeting DNA Vaccine pcDNA3/MDC-VP1 and its Immunological Effect on Mice[J].Journal of Hebei University (Natural Science Edition),2006,26(1):16-20. |
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| Authors: | WANG Xiao-ling WU Shu-hui GAO Zhi-yun WANG Yong-xiang |
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| Abstract: | Macrophage-derived chemokine(MDC) gene fragment was amplified by RT-PCR,and linked to Coxsackievirus B3(CVB3) VP1 gene to construct an eukaryotic expressing plasmid pcDNA3/MDC-VP1 by a DNA sequence encoding a flexible polypeptide(10 amino acids).The plasmid was inoculated to mice as a vaccine.After the third inoculation,the mean neutralizing antibody titer in pcDNA3/MDC-VP1 group was higher than that of pcDNA3/VP1,and the virus titer of blood in pcDNA3/MDC-VP1 group was lower than that of pcDNA3/VP1.After 10 LD-(50) CVB3 challenge,the survival rate of pcDNA3/MDC-VP1 group was 50%,Kaplan-Meier test was used to compare differences of survival curves,the results showed that the survive state of pcDNA3/MDC-VP1 group is better than that of other groups(P<0.05). |
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| Keywords: | macrophage-derived chemokine coxsackievirus B3 fusion gene neutralizing antibody mouse |
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