| 用渗透压冲击法提取青霉素酰化酶 |
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| 引用本文： | 张贺迎,武金霞,周艳芬,潘延云,姚翠鸾.用渗透压冲击法提取青霉素酰化酶[J].河北大学学报(自然科学版),2000,20(1):63-65. |
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| 作者姓名： | 张贺迎 武金霞 周艳芬 潘延云 姚翠鸾 |
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| 作者单位： | 1. 河北大学,生物工程研究所,河北,保定,071002
2. 河北大学,生命科学学院,河北,保定,071002 |
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| 摘 要： | 采用渗透压冲击法提取青霉素酰化酶 ,粗酶液比活达8.20×10-8 mol/(s·mg) ,比超声波法高10倍以上 ,酶活总收率达93.3 %。
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| 关 键 词： | 青霉素G酰化酶 渗透压冲击法 提取 |
| 文章编号： | 1000-1565(2000)01-0063-03 |
| 修稿时间： | 1999-05-06 |
Penicillin Acylase Extraction by Osmotic Shock Method |
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| ZHANG He-ying,WU Jin-xia,ZHOU Yan-fen,PAN Yan-yun,YAO Cui-luan.Penicillin Acylase Extraction by Osmotic Shock Method[J].Journal of Hebei University (Natural Science Edition),2000,20(1):63-65. |
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| Authors: | ZHANG He-ying WU Jin-xia ZHOU Yan-fen PAN Yan-yun YAO Cui-luan |
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| Institution: | ZHANG He-ying
(Institute of Bioteehnology,Hebei University,Baoding 071002,China)WU Jin-xia
(College of Life Science,Hebei University,Baoding 071002,China)ZHOU Yan-fen
,PAN Yan-yun
(Institute of Bioteehnology,Hebei University,Baoding 071002,China)YAO Cui-luan
(College of Life Science,Hebei University,Baoding 071002,China) |
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| Abstract: | Penicillin acylase was extracted by osmotic shock method. The specific activity of crude extract was 8.2×10-8 mol/(s·mg),it was ten-times higher than that from ultrasonic method.The total recovery was 93.3%. |
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| Keywords: | penicillin acylase osmotic shock extraction |
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