| 人工合成蚯蚓29肽的特性 |
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| 引用本文： | 赵晓瑜,李国建,倪志华,李晓霞.人工合成蚯蚓29肽的特性[J].河北大学学报(自然科学版),2009,29(1):76. |
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| 作者姓名： | 赵晓瑜 李国建 倪志华 李晓霞 |
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| 作者单位： | 1.河北大学,生命科学学院,生物工程技术研究中心,河北,保定,071002； 2.河北省迁安市马兰庄镇,农业办公室,河北,迁安,064405 |
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| 基金项目： | 河北省教育厅资助项目 |
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| 摘 要： | 为了解蚯蚓抗菌29肽的特性,采用菌落计数法对其抗菌谱、热稳定性、作用时间,以及最低抑制浓度等性质进行了研究.结果证实:该抗菌肽1)具有广谱性,即不仅有效抑制大肠杆菌、金黄色葡萄球菌等常见细菌,而且还能够抑制酿酒酵母、露湿漆斑菌、总状毛霉等真菌;2)活力高,当质量浓度为0.009 g/L时对大肠杆菌就有明显抑制效果;3)稳定性强,在pH为2.2或11的环境中抑菌活性不变,而且在煮沸10 min后仍能保留80%以上的活性.
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| 关 键 词： | 蚯蚓 抗菌肽 抗菌谱 最低抑菌质量浓度 |
Properties of Synthesized 29aa-Peptides from Lumbricus rubellus |
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| ZHAO Xiao-yu,LI Guo-jian,NI Zhi-hua,LI Xiao-xia.Properties of Synthesized 29aa-Peptides from Lumbricus rubellus[J].Journal of Hebei University (Natural Science Edition),2009,29(1):76. |
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| Authors: | ZHAO Xiao-yu LI Guo-jian NI Zhi-hua LI Xiao-xia |
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| Institution: | 1.College of Life Sciences;Hebei University research center of bioengineering and technology;Baoding 071002;China;2.Agricultural Office of Malanzhuang Town in Qia'an;Qia'an 064405;China |
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| Abstract: | To understand the characteristics of 29aa-peptides from Lumbricus rubellus,the research used colony forming unit technique to study antimicrobial spectrum,thermostability,action time,and minimum inhibitory concentration(MIC) of 29aa-peptides.The results showed:1) It was a wide antimicrobial spectrum,not only inhibiting common bacterias such as Escherichia coli,Staphylococcus areus but also fungi such as Saccharomyces cerevisiae,Myrothecium roridum etc.;2) Its antimicrobial activity was so powerful that a co... |
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| Keywords: | Lumbricus rubellus antimicrobial peptide antimicrobial spectrum minimum inhibitory mass concentration |
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