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| 自制纤维素金属螯合亲和膜的部分吸附性能及纯化牛血SOD | |
| 引用本文： | 刘建荣,张昊,顾雅君,赵晓瑜.自制纤维素金属螯合亲和膜的部分吸附性能及纯化牛血SOD[J].河北大学学报(自然科学版),2009,29(4). |
| 作者姓名： | 刘建荣 张昊 顾雅君 赵晓瑜 |
| 作者单位： | 1. 河北省生物工程技术研究中心,河北,保定,071002 2. 河南双汇投资发展股份有限公司,河南,漯河,462000 3. 河北大学,生命科学学院,河北,保定,071002 |
| 基金项目： | 河北省科技研究与发展指导计划项目，河北省教育厅科学研究计划项目 |
| 摘 要： | 自制纤维素金属螯合亲和膜,研究亲和膜对金属离子(Cu2+,Ni2+,Fe3+)和蛋白质的吸附性能,建立一种简便实用的纯化SOD的方法.以纤维素滤纸为原料,经交联制成可螯合金属离子的纤维素亲和膜,筛选确定与亲和膜吸附量最大的金属离子,并以卵清蛋白的吸附-解吸实验考查该膜对蛋白质的吸附性能.结果,在Cu2+,Ni2+和Fe3+中,亲和膜对Cu2+的吸附量最大,多次重复实验Cu2+吸附量稳定维持在4.60×10-6mol/cm2左右.Cu2+亲和膜对生理盐水溶卵清蛋白的吸附量高于无离子水溶卵清蛋白,可达113×10-6g/cm2,但蛋白解吸率前者低于后者,表明一定浓度的NaCl有助于亲和膜对蛋白质的吸附,同时也使被吸附的蛋白的解吸率有所降低.吸附-解吸方式(静态法)分离纯化SOD,解吸样品比活达8770 U/mg,纯度为81%;吸附-洗脱方式(动态法)分离纯化SOD,穿柱流出样品比活达12418 U/mg,纯度为85%.自制纤维素亲和膜有较好的吸附性能和纯化效果,重复使用性能良好,制备和纯化过程简便易行,具有广阔应用前景. |
| 关 键 词： | 亲和膜 金属螯合 纤维素 超氧化物歧化酶(SOD) |
Partial Adsorption Characteristics of Self-made Cellulose Metal-chelated Affinity Membraneand Its Application for Purification of Bovine SOD from Blood |
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| LIU Jian-rong,ZHANG Hao,GU Ya-jun,ZHAO Xiao-yu.Partial Adsorption Characteristics of Self-made Cellulose Metal-chelated Affinity Membraneand Its Application for Purification of Bovine SOD from Blood[J].Journal of Hebei University (Natural Science Edition),2009,29(4). | |
| Authors: | LIU Jian-rong ZHANG Hao GU Ya-jun ZHAO Xiao-yu |
| Institution: | 1.Hebei Province Bioengineering Technological Research Center;Baoding 071002;China;2.Henan Shuanghui Investment and Development Company Limited;Luohe 462000;3.College of Life Sciences;Hebei University;China |
| Abstract: | To study adsorption characteristics for metal ion(Cu2+,Ni2+,Fe3+) and proteins of self-made cellulose metal-chelated affinity membrane,and establish an efficient and convenient method for purifing superoxide dismutase(SOD),affinity membrane with Cu2+,Ni2+ and Fe3+ was prepared using cellulose filter paper as a matrix.Adsorption characteristics for proteins of affinity membrane were studied by adsorption-desorption experiments of ovalbumin.The adsorption capacity of affinity membrane for Cu2+ was the largest... |
| Keywords: | affinity membrane metal chelating cellulose superoxide dismutase(SOD) |
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