| 洋葱染色体HSG法C显带重复性和成功率的研究 |
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| 引用本文： | 张洪达,朱燕祥,王继孝,燕克勤.洋葱染色体HSG法C显带重复性和成功率的研究[J].河北大学学报(自然科学版),1990(3). |
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| 作者姓名： | 张洪达 朱燕祥 王继孝 燕克勤 |
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| 作者单位： | 河北大学生物系
(张洪达，朱燕祥，王继孝)，河北大学生物系(燕克勤) |
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| 摘 要： | 本文研究了洋葱根尖染色体HSG法C显带的重复性与片龄的关系。我们掌握了不同烤片时间洋葱染色体标本在47℃0.25mol/lHCl条件下C显带规律,并找到C显带重复性及成功率高达96.47％的最佳烤片时间为78—126小时(60℃)及各种分带条件与重复性成功率的关系。并讨论了影响C显带成功率的各项因素。
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| 关 键 词： | C显带 HSG法 重复性 成功率 洋葱 |
Study on Chromosome C-Band ing Repeatafility and Successful Rate of Allium by HSG Method |
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| Zhang Hongda Zhu Yanxing Wang Jixiao Yan Keqin.Study on Chromosome C-Band ing Repeatafility and Successful Rate of Allium by HSG Method[J].Journal of Hebei University (Natural Science Edition),1990(3). |
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| Authors: | Zhang Hongda Zhu Yanxing Wang Jixiao Yan Keqin |
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| Institution: | Department of Biology |
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| Abstract: | This paper studies the selation between repeatafility of succeseful rale and film state on allium cope root tip chromosome C-banding by using HSG method. We contorted the C-banded law of allium cope sample on different roosted film state under 47℃ and 0.25mol/l HC1 condition and found the best roast fielm state in 78-126hr (60℃ ) on C-banding to be 96.47%. We found the relation between repeatafility successful rate and other banding condition the facters infuencing the successful rate of C-banding in studies . |
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| Keywords: | Allium cope C-banding HSG method Repeatafility successful rate |
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