| 红豆杉离体胚培养快速育苗研究 |
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| 引用本文： | 梅兴国,温川蓉,刘凌,柯铁.红豆杉离体胚培养快速育苗研究[J].华中科技大学学报(自然科学版),1998(5). |
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| 作者姓名： | 梅兴国 温川蓉 刘凌 柯铁 |
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| 作者单位： | 华中理工大学生物工程系 |
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| 基金项目： | 国家科委“八五”重点科技攻关资助项目,国家科委“九五”重点科技攻关资助项目 |
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| 摘 要： | 采用Ｂ５和ＭＳ相组合的ＢＭ基本培养基和适宜浓度的激素（２，４－Ｄ，６－ＢＡ和ＧＡ３）能解除红豆杉种胚的休眠．单因子试验和不同组合的正交试验结果显示：单独使用２，４－Ｄ０．１ｍｇ／Ｌ，６－ＢＡ２ｍｇ／Ｌ和ＧＡ３２ｍｇ／Ｌ均在一定程度上促使红豆杉种胚的萌发，萌发率分别为７０％，５５％和２８．６％；最佳正交组合为６－ＢＡ１ｍｇ／Ｌ＋２，４－Ｄ０．１ｍｇ／Ｌ＋ＡＣ４ｇ／Ｌ，萌发率达８１％．
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| 关 键 词： | 红豆杉 胚培养 破除休眠 |
Studies on the Seedling Culture of Yew Embryo in Vitro |
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| Mei Xingguo Prof., Dept. of Biological Eng.,HUST,Wuhan ,China. Wen Chuanrong Liu Ling Ke Tie.Studies on the Seedling Culture of Yew Embryo in Vitro[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,1998(5). |
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| Authors: | Mei Xingguo Prof Dept of Biological Eng HUST Wuhan China Wen Chuanrong Liu Ling Ke Tie |
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| Institution: | Mei Xingguo Prof., Dept. of Biological Eng.,HUST,Wuhan 430074,China. Wen Chuanrong Liu Ling Ke Tie |
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| Abstract: | The dormancy of Yew embryo (T. chinesis var meirei ) was broken effectively by means of embryo culture in vitro in the modified medium. Experiments of single factor and orthogonal tests showed that using 2.4 D 0.1mg/L, 6 BA 2mg/L, or GA 3 2mg/L individually could enhance the germination of Yew embryo. The germination rates were 70%, 55%, 28.6% respectively. The best combination of the hormones was 6 BA 1mg/L, 2.4 D 0.1mg/L and AC 4g/L, and the best germination rate was 81%. |
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| Keywords: | Taxus chinesis var meirei embryo culture breaking of dormancy |
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