| 激素对小麦愈伤组织诱导和植株再生的影响 |
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| 引用本文： | 林刚,何勇刚,刘勇,何光源.激素对小麦愈伤组织诱导和植株再生的影响[J].华中科技大学学报(自然科学版),2004,32(9):111-113. |
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| 作者姓名： | 林刚 何勇刚 刘勇 何光源 |
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| 作者单位： | 华中科技大学,中英HUST-RRes作物基因工程与基因组学联合实验室,湖北,武汉,430074 |
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| 基金项目： | 国家转基因植物研究与产业化开发专项资助项目 (J99 B 0 0 9) |
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| 摘 要： | 比较了2，4-D和Picloram对小麦愈伤组织诱导和植株再生的影响．实验结果表明，培养基中加入2，4-D的诱导效果优于Picloram，2，4-D加入量为6mg／L时，诱导的愈伤组织的分化效果最好．对不同小麦品种幼穗愈伤组织诱导和植株再生的比较结果表明，鄂恩1号、鄂麦11、鄂麦12和山农1355等有较高的植株再生率。其中前3个材料植株再生率达90％以上；从幼穗、幼胚和成熟胚的比较结果来看，幼穗的愈伤组织诱导率和植株再生频率高于幼胚的和成熟胚．
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| 关 键 词： | 小麦组织培养 基因型 植株再生 外植体 |
| 文章编号： | 1671-4512(2004)09-0111-03 |
| 修稿时间： | 2003年8月29日 |
The effects of plant growth regulator and explants on wheat callus induction and regeneration |
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| Lin Gang He Yonggang Liu Yong He Guangyuan Senior Engineer, China UK HUST RRes Joint Lab.,Huazhong Univ. of Sci. & Tech.,Wuhan ,China..The effects of plant growth regulator and explants on wheat callus induction and regeneration[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2004,32(9):111-113. |
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| Authors: | Lin Gang He Yonggang Liu Yong He Guangyuan Senior Engineer China UK HUST RRes Joint Lab Huazhong Univ of Sci & Tech Wuhan China |
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| Institution: | Lin Gang He Yonggang Liu Yong He Guangyuan Senior Engineer, China UK HUST RRes Joint Lab.,Huazhong Univ. of Sci. & Tech.,Wuhan 430074,China. |
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| Abstract: | It was found that 2,4 D was better than Picloram in inducing callus from the explants and the best concentration was 6?mg/L. Inflorescence cultures of different varieties showed that EN1, EM11, EM12 and SN1355 had high frequencies of callus induction and plant regeneration, and the plant regeneration frequencies of the first three varieties was over 90%. Inflorescence cultures possessed higher frequencies of embryogenesis and plant regeneration than immature scutellum cultures and mature scutellum did. |
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| Keywords: | wheat tissue culture genotype regeneration explant |
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