秋茄和木榄耐盐内生细菌筛选及鉴定

为了改良和利用盐碱地，以秋茄(Ｋａｎｄｅｌｉａ ｏｂｏｖａｔａ)和木榄(Ｂｒｕｇｕｉｅｒａ ｇｙｍｎｏｒｈｉｚａ)为原材料，对耐盐内生细菌进行分离 并鉴定。 结果表明:从 ２ 种植物的根、茎、叶中初步分离出耐盐的内生细菌 ９６ 株，均为革兰氏阳性菌；提取各菌株的 ＤＮＡ 并扩 增其 １６Ｓ ｒＤＮＡ 序列，以 ＨｉｎｆⅠ、ＨａｅⅢ、ＭｓｐⅠ和 ＴａｑⅠ４ 种酶进行酶切，并统计遗传型，选取不同组合类型的代表菌株进行 １６Ｓ ｒＤＮＡ序列测定，通过 ＢＬＡＳＴ 比对分析并利用 ＭＥＧＡ 软件建立发育树等分子鉴定手段初步推断，所分离的内生细菌分别 为越南芽孢杆菌(Ｂａｃｉｌｌｕｓ ｖｉｅｔｎａｍｅｎｓｉｓ)、阿耶波多氏芽孢杆菌(Ｂａｃｉｌｌｕｓ ａｒｙａｂｈａｔｔａｉ)、芽孢杆菌属(Ｂａｃｉｌｌｕｓ ｓｐ.)、白翎芽孢杆菌 (Ｂａｃｉｌｌｕｓ ｂａｅｋｒｙｕｎｇｅｎｓｉｓ)和巴氏葡萄球菌(Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ｐａｓｔｅｕｒｉ)；对这 ５ 种菌的脱氨酶活性进行测定，越南芽孢杆菌的值最高。

Screening and Identification of Salt-tolerant Bacteria in Kandelia obovata and Bruguiera gymnorhiza

In order to ameliorate and utilize saline alkali soil,endophytes of kandelia obovata and Bruguiera gymnorhiza were isolated and identificated by combining physiological analysis and molecular identification.The results showed that 96 strains of salt-tolerant endophytic bacteria were preliminarily isolated from the roots and leaves of two plants,all of which were Gram-positive bacteria.The DNA of each strain was extracted,16S rDNA sequence by PCR,four enzymes were used for enzyme digestion,and the genetic types were counted.16S rDNA sequence determination of representative strains of different combination types was carried out,blast analysis,phylogenetic tree using establishing by MEGA software,the isolated endophytic bacteria were Bacillus vietnamensis,Bacillus aryabhattai,Bacillus baekryungensis,Bacillus ceurus and Staphylococcus pasteuri.The ACC deaminase activity of these five bacteria was determined,and the value of Bacillus vietnamensis was the highest.