农杆菌介导转化水稻成熟胚影响因素研究

实验对蜀恢881、蜀恢527、中花9号等7个水稻品种的成熟胚为受体材料，研究了影响农杆菌转化水稻频率的几个重要因素，实验结果表明：CC培养基是籼稻成熟胚愈伤组织的最适诱导培养基，而NB培养基是粳稻品种成熟胚的最适诱导培养基；诱导培养基中添加ABA并不能显著提高籼稻和粳稻成熟胚愈伤组织的诱导率，但在ABA为3mg／LN可以明显改善愈伤质量；适当的低温预处理可以不同程度提高诱导率；农杆菌菌株EHA105对水稻愈伤组织的转化能力优于LBA4404和AGL1；在分化时，采用适宜的激素配比有利于提高分化率。

Study on Some Factors Influencing Agrobacterium-Mediated Transformation of the Mature Embryos of Rice

Several impotant factors influencing transformation frequency of rice mediated by Agmbacterium were researched with seven indica and japonica rice cultivars such as SH881,SH527,ZH9 and so on.For indica rice,CC medium is the best for callus initiation.For japonica rice,NB medium is the best for callus initiation.Lead ABA in the culture not to improve the initiation rate of the mature embryo of indica rice and japonica rice notably, But can obviously improve and injure quality in ABA for 3mg/L; The proper low-temperature pretreatment can improve the initiation rate; The transformation ability of Agrobacterium strain EHA105 is superior to LBA4404 and AGL1 to rice; While differention , it helps to improve the differentiation rate to adopt suitable hormone combination.