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筛选假单胞菌株M18防治大棚黄瓜枯萎病害
引用本文:许煜泉,唐玮宁,郑有丽,钟仲贤,徐悌惟.筛选假单胞菌株M18防治大棚黄瓜枯萎病害[J].上海交通大学学报,1999,33(2):210-213.
作者姓名:许煜泉  唐玮宁  郑有丽  钟仲贤  徐悌惟
作者单位:1. 上海交通大学汇成健康科技研究所 200243
2. 上海市农业科学院园艺研究所 200243
基金项目:上海市科技兴农重点攻关资助项目
摘    要:设计了一种新的高效筛选程序,从植物根际土壤中分离出一株具有ACC脱氨酶活性的促生拮抗双功能假单胞菌株M18.在培养皿中,M18能有效地抑制黄瓜枯萎病菌以及多种其他枯萎病原菌和植物病原菌的生长.M18活菌浸种处理春黄瓜种子以及在大棚定植后根际土壤浇灌的试验中,与对照组相比,黄瓜枯萎病的株发病率降低70%~80%,霜霉病的发病率和病情指数都同时平均下降70%左右,黄瓜产量提高20%以上,每公顷可增加产值0.9万元以上,M18的生物防治效果达到极显著程度.

关 键 词:生物防治  假单胞菌株M18  ACC脱氨酶  黄瓜枯萎病
修稿时间:1998-08-27

Screening of Pseudomonas M18 and Its Biological Control of Cucumber Wilt Disease under Commercial Greenhouse
Xu Yuquan,Tang Weining,Zheng Youli,Zhong Zhongxian,Xu Tiwei.Screening of Pseudomonas M18 and Its Biological Control of Cucumber Wilt Disease under Commercial Greenhouse[J].Journal of Shanghai Jiaotong University,1999,33(2):210-213.
Authors:Xu Yuquan  Tang Weining  Zheng Youli  Zhong Zhongxian  Xu Tiwei
Abstract:A novel high efficiency procedure was designed and adopted for screening bifunctional Pseudomonas sp.M18. This strain has 1 aminocyclopropane 1 carboxylate (ACC) deaminase activity and was considered to be a plant growth promoting rhizobacterium. And it was demonstrated to has strong suppression activity against Fusarium spp., as well as several other fungal phytopathogens. In the field trial under commercial greenhouse condition, cucumber seeds were treated with M18 suspension, then the cucumber rhizosphere was treated with 1/100 diluted suspension after transplanting. It was found that M18 could preferentially colonize 10 times more in the rhizosphere than in the soil of surface layer. The systemic disease resistance was induced in the M18 protected cucumber plant. Compared with CK group, average incidence of wilt disease dropped down 70%-80%, and disease index of Downy Mildew also reduced by 70%. As a result, the cucumber yield was increased by 24% more. The biological control efficiency reached a very significant level according to the test.
Keywords:biological control  Pseudomonas  M18  ACC deaminase  cucumber  Fusarium  wilt disease  
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