| 异色瓢虫对3种杀虫剂抗性选育过程中酯酶活性和蛋白含量的动态变化 |
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| 引用本文： | 袁瑞,杨洪.异色瓢虫对3种杀虫剂抗性选育过程中酯酶活性和蛋白含量的动态变化[J].贵州科学,2012,30(4):29-33. |
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| 作者姓名： | 袁瑞 杨洪 |
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| 作者单位： | 1. 铜仁职业技术学院生物工程系,铜仁,554300
2. 贵州大学昆虫研究所贵州山地农业病虫害重点实验室,贵阳,550025 |
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| 摘 要： | 本文研究了异色瓢虫对3种杀虫剂(高效氯氰菊酯、吡虫啉、毒死蜱)抗性选育过程中酯酶活性和蛋白含量的动态变化。研究结果表明,随着选育代数的增加,酯酶活性和蛋白含量也有所增加,高效氯氰菊酯选育的异色瓢虫酯酶活性增大的倍数较另外两种大,而经3种杀虫剂选育的异色瓢虫蛋白含量增加的不明显。研究结果可为进一步研究捕食性瓢虫的抗逆性机制提供借鉴。
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| 关 键 词： | 异色瓢虫 酯酶活性 蛋白含量 |
Dynamic Variation of Esterase Activity and Protein Content in Resistance Selec- tion Processes of Harmonia axyridis to Three Kinds of Insecticides |
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| YUAN Rui,YANG Hong.Dynamic Variation of Esterase Activity and Protein Content in Resistance Selec- tion Processes of Harmonia axyridis to Three Kinds of Insecticides[J].Guizhou Science,2012,30(4):29-33. |
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| Authors: | YUAN Rui YANG Hong |
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| Institution: | 1 Bioengineering Department of Tongren Vocational ＆ Technical College, Tongren, Guizhou 554300, Chi- na;elnstitute of Entomology, Guizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guizhou University, Guiyang , Guizhou 550025, China) |
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| Abstract: | This paper studied dynamic variation of the esterase activity and protein content in resistance selection processes of Harmonia axyridis to beta-cypermethrin, imidacloprid and chlorpyrifos. The results showed that enzyme activity and protein content increased with breeding generations increased; cypermethrin breeding strain esterase activity increased multiples higher than the other two kinds; and the three kinds of insecticides in breeding strains protein content increased not obviously. The research results could provide references to further work on resistance mechanism of predatory ladybeetles. |
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| Keywords: | Harmonia axyridis esterase activity protein content |
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