中国毛虾木瓜蛋白酶水解的工艺条件

以水解度和总氮回收率为指标，研究了单酶水解中国毛虾的工艺条件，分析了酶解温度、加酶量、料水比、酶解时间四因素对中国毛虾蛋白水解的影响。通过正交试验，确定最佳水解条件。综合考虑，木瓜蛋白酶水解中国毛虾的最适条件为：温度55℃，加酶量0．5％，料水比1：4，水解时间3h。在此条件下，中国毛虾的水解度为30．69％，总氮回收率为68．82％。

Study on Enzymatic Hydrolysis to Acetes Chinensis Bypapain

Using the degree of hydrolysis and total nitrogen recovery as targets, non-enzymatic hydrolysis of Acetes Chinensis protein was studied in this paper. The effects of the main parameters including enzymatic temperature, enzymatic dosage, the weight radio of solvent to solute, and enzymatic time on hydrolysis reaction were investigated. On the basis of orthogonal tests, the optimum hydrolytic conditions were determined. In a word,the suitable hydrolysis conditions were that hydrolysis temperature was 55℃, enzyme dosage 0.5%, the ratio of solid to water 1 ： 4 and hydrolysis duration 3 h. Under these conditions, the degree of hydrolysis and total nitrogen recovery were 30.69% and 68.82% respectively.