| 黄孢原毛平革菌锰过氧化物酶基因（MnP2）的克隆 |
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| 引用本文： | 杨晓宽.黄孢原毛平革菌锰过氧化物酶基因（MnP2）的克隆[J].河北科技师范学院学报,2009,23(4):45-49. |
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| 作者姓名： | 杨晓宽 |
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| 作者单位： | 河北科技师范学院,食品科技学院,河北,秦皇岛,066600 |
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| 摘 要： | 应用RT-PCR技术,从黄孢原毛平革菌5.766（Phanerochaete chrysosporium）总RNA中成功扩增出预期大小约为1.3 kb的特异性条带,将扩增产物提纯后克隆入PUC19载体,经转化、筛选及酶切鉴定后,获得锰过氧化物酶（MnP2）基因的克隆。序列分析表明,扩增的MnP2基因片段其cDNA长度为1 149 bp,编码358个氨基酸,与其它已发表文献报道的MnP2序列一致,与其同工酶MnP1和MnP3的核苷酸同一性分别为81%和66%。
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| 关 键 词： | 锰过氧化物酶 cDNA 同工酶序列 反转录聚合酶链式反应 |
Molecular Clone of cDNA Encoding Manganese Peroxidase(MnP2) from Phanerochaete chrysosporium |
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| YANG Xiao-kuan.Molecular Clone of cDNA Encoding Manganese Peroxidase(MnP2) from Phanerochaete chrysosporium[J].Journal of Hebei Normal University of Science & Technology,2009,23(4):45-49. |
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| Authors: | YANG Xiao-kuan |
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| Institution: | YANG Xiao-kuan (School of Food Science and Engineering, Hebei Normal University of Science ＆ . Technology, Qinhuangdao Hebei, 066004, China) |
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| Abstract: | cDNA encoding specialized fragment about 1. 3 kb was amplified successfully by RT-PCR from the total RNA of Phanerochaete chrysosporium 5. 776 and cloned into puc19 vector for sequence after purification. The MnP2 gene cloning was obtained after transformation, screening and enzyme-cut identification. The sequence analysis showed that the cDNA nucleotide sequence of the MnP2 was 1 149 bp and encoded a protein of 358 amino acids. Sequence comparison with other published MnP2 genes showed that the nucleotide identity was 100% while sequence comparison with MnP1 and MnP3 gene Showed that the nucleotide identity was respectively 81% and 66%. |
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| Keywords: | cDNA |
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