| N-乙酰氨基葡萄糖对绛红小单孢菌合成庆大霉素的影响 |
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| 引用本文： | 管玉霞,孙甲贤,王静.N-乙酰氨基葡萄糖对绛红小单孢菌合成庆大霉素的影响[J].中国科学技术大学学报,2011(11). |
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| 作者姓名： | 管玉霞 孙甲贤 王静 |
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| 作者单位： | 福州大学生物科学与工程学院; |
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| 基金项目： | 福建省重点项目(2008Y0052)资助 |
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| 摘 要： | 研究了N-乙酰氨基葡萄糖对绛红小单孢菌产素的影响,实验探索了N-乙酰氨基葡萄糖添加工艺,在250mL摇瓶发酵过程中培养48h,60h分别添加0.05%,0.15%N-乙酰氨基葡萄糖可使庆大霉素生测效价达2 030U/mL,较对照1 228U/mL提高了65%以上(三次平均基本一致);5L发酵罐试产三批次发酵终结,生测效价平均为1 727U/mL(厡工艺1 097U/mL)提高了57%,效果十分明显.同时测定了发酵过程中菌体代谢各种参数的变化,证明了N-乙酰氨基葡萄糖在绛红小单孢菌生物合成中的促进作用.本方法操作简单,基本不改变原工艺,生产成本低廉,对提高庆大霉素产量效果非常明显.
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| 关 键 词： | 绛红色小单孢菌 庆大霉素 N-乙酰氨基葡萄糖 生测效价 代谢参数 |
Effect of N-acetyl glucosamine on the production of gentamicin in Micromonospora purpurea |
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| GUAN Yuxia,SUN Jiaxian,WANG Jing.Effect of N-acetyl glucosamine on the production of gentamicin in Micromonospora purpurea[J].Journal of University of Science and Technology of China,2011(11). |
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| Authors: | GUAN Yuxia SUN Jiaxian WANG Jing |
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| Institution: | GUAN Yuxia,SUN Jiaxian,WANG Jing(College of Biological Science and Technology,Fuzhou University,Fuzhou 350002,China) |
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| Abstract: | The effects of N-acetyl glucosamine(N-AGA)on the production of Gentamicin in Micromonospora purpurea were researched and adding process of N-AGA was explored.It was found that when N-AGA was added into the fermentation with the concentration of 0.05% and 0.15% after 48 h and 60 h of fermentation respectively in a 250 mL flasks,the biological titer was 2 030 U/ml and increased by over 65% compared to control 1 228 U/mL;the average biological titer of the 3 batches was 1 727 U/mL in 5 L fermentor and was incr... |
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| Keywords: | Micromonospora purpurea gentamicin N-acetyl glucosamine biological titer metabolic parameter |
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