| P(3-co-4)HB解聚酶的分离纯化及酶学性质研究 |
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| 引用本文： | 陈珊,郭子琦,李凡,刘东波,夏红梅.P(3-co-4)HB解聚酶的分离纯化及酶学性质研究[J].大庆师范学院学报,2011(6):96-100. |
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| 作者姓名： | 陈珊 郭子琦 李凡 刘东波 夏红梅 |
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| 作者单位： | 东北师范大学生命科学学院; |
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| 基金项目： | 吉林省科技发展计划资助项目(20090594) |
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| 摘 要： | 聚(3-羟基丁酸酯-co-4-羟基丁酸酯)(P(3-co-4)HB)解聚酶是由微生物所分泌的一种胞外酶,可以将P(3-co-4)HB降解为水溶性小分子物质,为了阐明P(3-co-4)HB材料的生物降解机理并建立其生物循环系统,P(3-co-4)HB降解菌和降解酶的研究越来越受到关注。本论文以Penicillium sp.DS-04菌株为材料,对其产生的P(3-co-4)HB解聚酶进行了分离纯化,发酵液经过冻干和分子筛层析得到了P(3-co-4)HB解聚酶的纯酶组分,纯化倍数和回收率分别为3.15和10.44%,SDS-PAGE显示该酶分子量为44.0kDa。本文还对P(3-co-4)HB解聚酶的酶学性质进行了测定,并初步探讨了解聚酶的作用模式。
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| 关 键 词： | Penicilliumsp.DS-04 聚(3-羟基丁酸酯-co-4-羟基丁酸酯)(P(3-co-4)HB) 解聚酶 |
Purification and Characterization of P(3 - co - 4) HB Depolymerase from Penicillium sp. DS - 04 |
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| CHEN Shan,GUO Zi-qi,LI Fan,LIU Dong-bo,XIA Hong-mei.Purification and Characterization of P(3 - co - 4) HB Depolymerase from Penicillium sp. DS - 04[J].Journal of Daqing Normal University,2011(6):96-100. |
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| Authors: | CHEN Shan GUO Zi-qi LI Fan LIU Dong-bo XIA Hong-mei |
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| Institution: | CHEN Shan,GUO Zi-qi,LI Fan,LIU Dong-bo,XIA Hong-mei(School of Life Science,Northeast Normal University,Changchun 130024,China) |
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| Abstract: | An extracellular poly-3-hydroxybutyrate-co-4-hydroxybutyrate depolymerase was purified from the culture supernatant of Penicillium sp.DS-04.Gel filtration chromatography was used to separated the depolymerase.The specific activity of the purified enzyme was increased by 3.15 folds over crude extract,and the recovery yield was 10.44%.SDS-PAGE analyses revealed a single band of 44.0kDa.The properties of the purified enzyme were measured and analyzed.The analysis of products after enzymatic degradation reveale... |
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| Keywords: | Penicillium sp DS-04 poly-3-hydroxybutyrate-co-4-hydroxybutyrate depolymerase |
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