| 平颏海蛇CRVP基因全长cDNA片段的克隆和序列分析 |
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| 引用本文： | 卫剑文,钟肖芬,彭立胜,吴文言,徐安龙.平颏海蛇CRVP基因全长cDNA片段的克隆和序列分析[J].中山大学学报(自然科学版),2001,40(4):5-8. |
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| 作者姓名： | 卫剑文 钟肖芬 彭立胜 吴文言 徐安龙 |
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| 作者单位： | 中山大学生命科学学院 |
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| 基金项目： | 国家高新技术海洋领域863资助项目(819-06-06) |
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| 摘 要： | 通过对平颏海蛇毒腺cDNA文库的随机测序和PCR筛选，克隆得到两种分别长为1309和1307bp的编码半胱氨酸丰富毒蛋白（cysteine-rich venom protein，CRVP）的全长cDNA序列。这两种crvp基因同源性为97％，分别编码238和199个氨基酸残基，其中包括一段相同的由19个氨基酸残基组成的信号肽。氨基酸序列分析表明，这两种CRVP蛋白与蜥蜴helothermine毒素蛋白以及台湾饭铲倩CRVP毒蛋白高度同源，而且具有半胱氨酸丰富分泌蛋白（cysteine-rich secretory protein,CRISP)家族的典型结构特征。
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| 关 键 词： | 平颏海蛇 半胱氨酸丰富毒蛋白 全长cDNA序列 基因克隆 序列分析 序列结构 |
| 文章编号： | 0529-6579(2001)04-0005-04 |
| 修稿时间： | 2000年9月1日 |
Cloning and Sequence Analysis of Full - Length cDNA Encoding Cysteine - Rich
Venom Protein from Lapemis hardwickii |
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| WEI Jian-wen,ZHON Xiao-fen,PENG Li-sheng,WU Wen-yan,XU An-long.Cloning and Sequence Analysis of Full - Length cDNA Encoding Cysteine - Rich
Venom Protein from Lapemis hardwickii[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2001,40(4):5-8. |
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| Authors: | WEI Jian-wen ZHON Xiao-fen PENG Li-sheng WU Wen-yan XU An-long |
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| Abstract: | Two full-length cDNA, 1 309 and 1 307 bp in length, for cysteine-rich venom protein (CRVP) were constructed by sequencing and PCR Screening of cDNA clones from a cDNA library of venom gland of Lapemis hardwickii. The sequence identity of two cDNAs was 97%. The predicted CRVP consisted of 238 and 199 amino acid residues including a putative signal peptide of 19 residue, and they were highly homologous to a lizard helother- mine and a Taiwan Habu CRVP. Amino acid sequence analysis suggested that the CRVP had the specific sequence structure, which was conserved in cysteine - rich secretory protein (CRISP) family. |
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| Keywords: | Lapemis hardwickii cysteine-rich venom protein |
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