| 一种简易的植物核酸提取方法─—从干叶和新鲜叶中快速提取RNA和DNA |
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| 引用本文： | 施苏华,章群,陈月琴,唐绍清,屈良鹄.一种简易的植物核酸提取方法─—从干叶和新鲜叶中快速提取RNA和DNA[J].中山大学学报(自然科学版),1996(2). |
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| 作者姓名： | 施苏华 章群 陈月琴 唐绍清 屈良鹄 |
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| 作者单位： | 中山大学生命科学学院 |
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| 基金项目： | 国家自然科学基金，国家教委博士点基金，中山大学科研基金 |
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| 摘 要： | 本文介绍了一种植物核酸提取的简易方法．该法只需在常温下按常规的分子生物学操作条件从已干燥的和新鲜的植物叶中快速提取总ＲＮＡ和ＤＮＡ．所获得的ＲＮＡ和ＤＮＡ能顺利用于下一步的ＰＣＲ、测序及小分子ＲＮＡ研究等分子生物学实验．
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| 关 键 词： | 植物核酸提取，硅胶干燥保存，RNA和DNA |
A Simple Method for Isolation of Total RNA and DNA from Silicagel-dried and Fresh Leaves of Plants |
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| Shi Suhua, Zhang Qun, Chen Yueqin, Tang Shaoqing, Qu Lianghu.A Simple Method for Isolation of Total RNA and DNA from Silicagel-dried and Fresh Leaves of Plants[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1996(2). |
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| Authors: | Shi Suhua Zhang Qun Chen Yueqin Tang Shaoqing Qu Lianghu |
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| Abstract: | A simple and rapid method was proposed for the isolation of total DNA and RNA from silicageldryed leaves as well as fresh leaves of various groups of plants. without special clearing treatments of solutions, glassware and plasticware, and the use of poisonous phenol. This method is fairely suitable for the isolation of DNA and RNA from collection of plant leaves dryed in silicagel in field Which is convenient for international exchange. The isolated total DNA and RNA can be applied effectively in PCR, sequencing. SnRNA studies and other molecular biological researches. |
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| Keywords: | efficient method isolation total DNA and RNA |
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