| 新基因Splt137在大肠杆菌中的表达及抗体制备 |
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| 引用本文： | 尹崇,李朝飞,王立华,张萍,庞义.新基因Splt137在大肠杆菌中的表达及抗体制备[J].中山大学学报(自然科学版),2002,41(4):75-77. |
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| 作者姓名： | 尹崇 李朝飞 王立华 张萍 庞义 |
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| 作者单位： | 中山大学生物防治国家重点实验室//昆虫学研究所,广东,广州,510275 |
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| 基金项目： | 国家“973”专题资助项目 (G2 0 0 0 0 16 2 2 0 9) |
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| 摘 要： | 用PCR的方法扩增得到斜纹夜蛾核多角体病毒 (SpltMNPV)ORF137全长基因 (Splt137)。将其克隆到原核表达载体pQE30上 ,并转化大肠杆菌M15 ,在IPTG诱导下表达了与预期相对分子质量相符的一个 2 7× 10 7的蛋白质 ,经过聚丙烯酰氨凝胶电泳纯化 ,免疫家兔制备了抗Splt137抗体。Western免疫印迹分析表明 ,该抗体适合用作Splt137蛋白的进一步分析。
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| 关 键 词： | 斜纹夜蛾核多角体病毒 Splt137 原核表达 抗体 |
| 文章编号： | 0529-6579(2002)04-0075-03 |
| 修稿时间： | 2002年4月17日 |
Expression of a New Gene Splt137 in E.coli and Preparation of Antiserum |
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| YIN Chong,LI Zhao_fei,WANG Li_hua,ZHANG Ping,PANG Yi.Expression of a New Gene Splt137 in E.coli and Preparation of Antiserum[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2002,41(4):75-77. |
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| Authors: | YIN Chong LI Zhao_fei WANG Li_hua ZHANG Ping PANG Yi |
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| Abstract: | One of Spodoptera litura multinucleocapsid nuclear polyhdrosis virus ( Splt MNPV) unique ORFs ORF137 ( Splt137 ) was obtained by PCR method from genome DNA. The PCR products were cloned into the pGEM_T easy vector to get the recombinant plasmid (pGT_SL137). The Splt 137 was recombined in vitro with expression vector pQE30 and transformed into E coli M15. The M15 strain, containing Splt137 recombinant plasmid, expressed a 27 000 protein in agreement with the prospected molecule after the induction with IPTG. The expressed protein was used to raise antiserum. Western blot analysis indicated that the antiserum could react with the corresponding protein, and was suitable to be used for further analysis of Splt137 protein. |
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| Keywords: | Splt MNPV Splt137 procaryotic expression antibody |
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