| 虫草属多元起源的分子生物学证据 |
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| 引用本文： | 王宁,陈月琴,章卫民.虫草属多元起源的分子生物学证据[J].中山大学学报(自然科学版),2000,39(4):70-73. |
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| 作者姓名： | 王宁 陈月琴 章卫民 |
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| 作者单位： | 中山大学生物工程研究中心!广东广州510275(王宁，陈月琴，屈良鹄)，广东省微生物研究所(章卫民，李泰辉) |
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| 基金项目： | 国家杰出青年基金资助项目!(3952 50 0 7)，国家自然科学基金资助项目!(39770 0 58) |
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| 摘 要： | 测定了虫草属 (Cordyceps)代表种及其相关无性型菌 (anamorphicstate )核糖体rDNAITS区序列 ,并从国际分子生物学数据库中调取相关序列 ,以Phylip35分析软件构建序列距离矩阵和分子系统发育树图 ,结果表明 :虫草属各个种之间ITS区序列差别比较大 ,不能形成一个单系的类群 .冬虫夏草 ,古尼虫草 ,蛹虫草 ,大团囊虫草在系统发育上分别与丝孢纲(Hyphomycetes) ,束梗孢目 (Stilbelaes)的中国被毛孢 ,丝孢目 (Hyphomycetales)的黄绿绿僵菌 ,丝孢目 (Hyphomycetales)的球孢白僵菌 ,担子菌纲的Cyphomyrmexminutus非常接近 ,在树图上分别处于相对独立的分支 ;说明形态学上划分的虫草属并非一自然发生的类群 ,也说明了虫生真菌形态与基因水平进化的不一致性 .
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| 关 键 词： | 虫草属(Cordyceps) rDNAITS区 多元起源 |
Molecular Evidences Indicating Multiple Origins in the Entomogenous Cordyceps |
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| WANG NingBiotechnology Research Center,Zhongshan University,Guangzhou ,China,CHEN Yue-qin,ZHANG Wei-min,LI Tai-hui,QU Liang-hu.Molecular Evidences Indicating Multiple Origins in the Entomogenous Cordyceps[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2000,39(4):70-73. |
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| Authors: | WANG NingBiotechnology Research Center Zhongshan University Guangzhou China CHEN Yue-qin ZHANG Wei-min LI Tai-hui QU Liang-hu |
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| Institution: | WANG NingBiotechnology Research Center,Zhongshan University,Guangzhou 510275,China,CHEN Yue-qin,ZHANG Wei-min,LI Tai-hui,QU Liang-hu |
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| Abstract: | The nucleotide sequences of rDNA ITS and 5 8S ribosome DNA from several representative species of Cordeceps were determined and compared with published sequences. Based on sequence data, the phylogenetic trees were constructed using the NJ and Fitch methord.Diversity in the phylogenetic tree was found for Cordyceps species,C.sinensis,C.Militaris,C.gunnii,C.ophioglossoide were closely related to Hirsutella sinensis, Beauveria bassiana,Metarhizjum flavovirde,Cyphomyrmex minutus respectively. rDNA ITS sequences analysis indicated Cordyceps species are not a monophyletic group, and presented new insight into traditional concepts of the genus Cordyceps. |
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| Keywords: | rDNA ITS Cordyceps phylogeny multiple origins |
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