| 超临界CO_2萃取珊瑚姜挥发油抗部分植物病原菌活性研究 |
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| 引用本文： | 高玉琼,杨迺嘉,刘建华,赵德刚,宋宝安.超临界CO_2萃取珊瑚姜挥发油抗部分植物病原菌活性研究[J].中山大学学报(自然科学版),2008(Z1). |
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| 作者姓名： | 高玉琼 杨迺嘉 刘建华 赵德刚 宋宝安 |
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| 作者单位： | 贵州大学教育部绿色农药与农业生物工程重点实验室//贵州省农业生物工程重点实验室;贵州省生物技术研究开发基地; |
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| 基金项目： | 贵州省长基金资助项目(自然科学类)[黔省专合字(2006)81] |
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| 摘 要： | 采用超临界CO_2萃取珊瑚姜挥发油,对其成分进行GC/MS分析,利用生长速率法测定珊瑚姜挥发油对水稻稻瘟、水稻纹枯及小麦赤霉病菌的抗菌活性,结果显示,珊瑚姜挥发油检出29个化学成分,含量最高的化合物为萜品烯-4-醇;珊瑚姜挥发油对小麦赤霉病菌的EC_(50)为247.80 mg/L(Y=3.7940X-4.0832,r= 0.970 5),水稻稻瘟病菌的EC_(50)为99.64 mg/L(Y=1.1651X+2.6716,r=0.9090),水稻纹枯病菌的EC_(50)为106.16 mg/L(Y=1.2878X+2.3909,r=0.9964),实验表明,珊瑚姜挥发油对供试菌株具有较强的抑制作用,显示了较好的应用开发前景。
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| 关 键 词： | 珊瑚姜 挥发油 超临界二氧化碳萃取 抗菌活性 气相色谱-质谱 |
Antifungal Activity of the Oil from Zingiber corallinum Hance by Supercritical CO_2 Extraction |
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| GAO Yu-qiong,YANG Nai-jia,LIU Jian-hua,ZHAO De-gang,SONG Bao-an.Antifungal Activity of the Oil from Zingiber corallinum Hance by Supercritical CO_2 Extraction[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2008(Z1). |
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| Authors: | GAO Yu-qiong YANG Nai-jia LIU Jian-hua ZHAO De-gang SONG Bao-an |
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| Institution: | GAO Yu-qiong~(1,2),YANG Nai-jia~2,LIU Jian-hua~2,ZHAO De-gang~1,SONG Bao-an~1 (1.Guizhou University,Key Laboratory of Green Pesticide , Agricultural Biological Engineering,Ministry of Education,Guizhou Key Laboratory of Agricutural Bioengineering,Guiyang 550025,China,2.Guizhou Institute of Biotechnology Research , Development,Guiyang 550002,China) |
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| Abstract: | The volatile oil from Zingiber corallinum,analyzed by GC-MS and identified with their mass spectra, were obtained by supercritical CO_2,whose antifungal activity were studied by using the method of growth rate.GC -MS analysis showed that twenty-nine components were characterized in the oil of Zingiber corallinum,the most constituent of which was Terpinen-4-ol,The antifungal activity of the oil were EC_(50)247.80 mg/L(Y= 3.7940X-4.0832,r=0.9705)to Fusarium graminearum,EC_(50)99.64mg/L(Y=1.1651X+2.6716,r= 0.9... |
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| Keywords: | Zingiber corallinum Hance volatile oil supercritical CO_2 extraction antifungal activity GC/MS |
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