甜菜夜蛾核多角体病毒（SeMNPV）gp 37基因的克隆及序列分析

通过鸟枪法克隆了甜菜夜蛾核多角体病毒（SeMNPV)基因组DNA EcoR I酶切的2．2kb片段，序列分析表明，该片段含有gp37基因，SeMNPV gp37基因的开放阅读框为801个核苷酸，编码268个氨基酸，预测蛋白质相对分子质量为30400，在gp37基因起始密码子上游有一典型的杆状病毒晚期基因启动子序列ATAAG，同其它昆虫杆状病毒和昆虫痘病毒GP37／Fusolin蛋白的比较结果表明，SeMNPV GP37与已知gp37/fusolin基因的氨基酸序列同源性较高（50－68％），在其蛋白序列内存在类似的保守区和可能的N－连接糖基化位点，在SeMNPV gp37基因下游存在一个完整阅读框和部分get基因序列。

Cloning and Sequencing of Spodoptera exigua Multicapsid Nucleopolyhedrovirus (SeMNPV) gp37 Gene

An EcoRⅠ 2.2 kb fragment of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) genome DNA was cloned by using random cloning method.The gp37 gene of SeMNPV which is contained in this fragment has been identified.The open reading frame of the SeMNPV gp37 gene is 801 nucleotides long encoding a putative protein of 268 amino acids with an Mr of about 30 400.The 5′-nonencoding region upstream of a translational start site contains one late consensus motif (ATAAG).The SeMNPV gp37 gene has 50～68 amino acid identity to other baculovirus gp37 genes and entomopoxvirus fusolin genes. Conserved regions of GP37/Fusolin very high amino acid conservation and potential N-linked glycosylation sites were also identified in SeMNPV GP37 sequence.