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ISSR标记在辣椒资源遗传多态性分析中的初步应用
引用本文:杨若林,孔俊,吴鑫,邓志瑞,陈沁,刘文轩.ISSR标记在辣椒资源遗传多态性分析中的初步应用[J].上海大学学报(自然科学版),2005,11(4):423-426,435.
作者姓名:杨若林  孔俊  吴鑫  邓志瑞  陈沁  刘文轩
作者单位:上海大学,生命科学学院,上海,200444
基金项目:上海市高等学校青年科学基金资助项目(207364)
摘    要:用筛选到的12个ISSR(InterSimpleSequenceRepeat)引物对我国11个辣椒农家品种进行扩增,结果共扩增出66条带,其中差异条带26条,多态条带比率(PPB)为39.39%;品种特异条带7条,占总条带数的10.61%.基于Jaccard遗传相似性系数的NeighborJoining聚类分析将11个品种分为两组,这两组辣椒品种内部的平均遗传距离分别为0.150和0.134,两组之间的遗传距离为0.194.以扩增条带表型为基础的主成分分析显示:有2个主成分累计贡献率为68.88%,分别占到57.51%和11.37%;以这2个主成分对11个辣椒品种作图,可以将11个辣椒品种分为两组,每组所含辣椒品种与聚类分析的结果相同.

关 键 词:辣椒  ISSR  聚类分析  主成分分析
文章编号:1007-2861(2005)04-0423-04
收稿时间:2004-07-02
修稿时间:2004年7月2日

Application of ISSR Markers in Genetic Polymorphism of Capsicum frutescens L.
YANG Ruo-lin,KONG Jun,WU Xin,DENG Zhi-rui,CHEN Qin,LIU Wen-xuan.Application of ISSR Markers in Genetic Polymorphism of Capsicum frutescens L.[J].Journal of Shanghai University(Natural Science),2005,11(4):423-426,435.
Authors:YANG Ruo-lin  KONG Jun  WU Xin  DENG Zhi-rui  CHEN Qin  LIU Wen-xuan
Abstract:DNA samples of 11 native cultivars of Capsicum were analyzed with 12 screened ISSR primers which produced polymorphisms. Out of a total of 66 amplified bands, 26 bands were divergent, accounting for 39.39%, and 7 of these 26 bands were cultivar-specific, that is 10.61%. The genetic dendrogram among the materials constructed by the Neighbor-Joining method based on the Jaccard coefficient showed that the 11 cultivars can be separated into two groups, one with an average genetic distance 0.150 and the other 0.134. The average genetic distance between the two groups were 0.194. The result of principal component analysis (PCA) based on the amplified bands revealed that two components had 68.33% cumulative contribution to the total variance with individual contributions of 57.51% and 11.37%, respectively. According to the two principal factors, the 11 cultivars were classified into two groups, each having the same cultivars as the groups in dendrogram.
Keywords:Capsicum  ISSR  phylogenic analysis  PCA
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