| 大鼠神经干细胞表达家蚕神经系统特异蛋白类似物 |
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| 引用本文： | 郭志华,沈琦,陈付学,文铁桥,宋红生.大鼠神经干细胞表达家蚕神经系统特异蛋白类似物[J].上海大学学报(自然科学版),2005,11(5):535-539. |
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| 作者姓名： | 郭志华 沈琦 陈付学 文铁桥 宋红生 |
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| 作者单位： | 上海大学生命科学学院,上海200444 |
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| 基金项目： | 国家教委留学回国基金资助项目（03AK64）;上海市高等学校科学技术发展基金资助项目（7A07221） |
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| 摘 要： | 用免疫组织化学的方法，研究了大鼠胎脑中家蚕神经系统特异蛋白（Bombyrin）的分布以及与神经干细胞的关系．结果表明，家蚕Bornbyrin类似物主要分布在大鼠胎脑的脑室下区、纹状体等区域，特别是表达在大鼠的神经干细胞中，而分化的神经干细胞则没有Bombyrin类似物的免疫活性．western blot的分析表明，大鼠脑中存在77kDa和110kDa 2种Bornbyrin类似的蛋白．这些结果暗示，大鼠脑中Bombyrin类似蛋白可能与神经干细胞特性的维持有关.
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| 关 键 词： | 大鼠 神经干细胞 免疫组织化学 家蚕 Bombyrin |
| 文章编号： | 1007-2861（2005）05-0535-05 |
| 收稿时间： | 2004-09-03 |
| 修稿时间： | 2004年9月3日 |
Like-Protein in Central Nerve System of Bombyx Silkworm Expressed in Neural Stem Cells of Fetal Rat |
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| GUO Zhi-hua, SHEN Qi, CHEN Fu-xue, WEN Tie-qiao, SONG Hong-sheng.Like-Protein in Central Nerve System of Bombyx Silkworm Expressed in Neural Stem Cells of Fetal Rat[J].Journal of Shanghai University(Natural Science),2005,11(5):535-539. |
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| Authors: | GUO Zhi-hua SHEN Qi CHEN Fu-xue WEN Tie-qiao SONG Hong-sheng |
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| Abstract: | Bombyrin is a protein, specifically expressed in the central nerve system of Bombyx silkworm. Using the Bombyrin antibody and nestin antibody, immuno-staining was performed on brain sections and culture cells of fetal rats. Bombyrin immunoreactivity was located in the cells of subventricular zones and the striatum region, and especially expressed in the neural stem cells, which were positive to nestin antibody, while Bombyrin immunoreactivity disappeared in the differentiated neural stem cells. Western blot analysis revealed that there were two kinds of Bombyrin-like protein (77 kDa and 110 kDa) in the neural stem cells of the rat. These results suggest that Bombyrin-like protein in the rat may be related to the maintenance of the neural stem cells. |
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| Keywords: | rat neural stem cell immunohistochemistry Bombyx rnori Bombyrin |
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