产广谱高效多烯类抗真菌抗生素的纺锤链霉菌SD-07（Streptomyces netropsis）的分离及鉴定

从山东省济南市南部山区土壤中分离得到1株具有广谱抗真菌活性的链霉菌SD-07菌株。根据形态特征、生理生化反应和16S rDNA序列分析结果，链霉菌SD-07鉴为定为纺锤链霉菌（Streptomyces netropsis）。分离纯化了SD-07产生的抗真菌活性成分并获得其结晶CA—SD07。抗真菌活性检测结果发现：CA-SD07对酵母菌、担子菌、接合菌等多种真菌具有广谱抗菌作用。对CA—SD07进行性质研究发现，抗真菌活性成分CA—SD07抗菌谱广且抗菌活性高（约等于2倍的两性霉素B），是戊烯和七烯混合的多烯大环内酯类抗真菌抗生素。

Isolation and classification of Streptomyces netropsis strain SD-07 which produces polyene macrolide antibiotics with broad-spectrum and high anti-fungal activity

Streptomyces sp. SD-07 was isolated from the soil of a mountain area in the south of Jinan, Shandong Province. This strain exhibited broad-spectrum and high antifungal activity. Based on the results of prehminary morphological, cultural, physiological and biochemical studies on this strain, and its 16S rDNA sequences, strain SD-07 was classified as Streptomyces netropsis. The antifungal compound CA-SD07 were extracted from the mycelia and then crystallized, and named as CA-SD07. CA-SD07 could inhibit many fungi such as Ascomycetes, Basidiomycetes and Zygomycetes. The data from ultraviolet-visible absorption and high-performance liquid chromatography / diode array detection （HPLC / DAD） indicated that CA-SD07 was a mixture of pentaene macmlide and heptaene macrolide antibiotics, and the major compound was pentaene macrolide. CA-SD07 has wider antifungal spectrum and higher antifungal activity（ about two times Amphotefic B）.