| 超螺旋PGEM-1重组质粒的提取、纯化与鉴定 |
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| 引用本文： | 杜培革,项小丰,付桂莲,李质华.超螺旋PGEM-1重组质粒的提取、纯化与鉴定[J].北华大学学报(自然科学版),1995(3). |
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| 作者姓名： | 杜培革 项小丰 付桂莲 李质华 |
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| 作者单位： | 吉林医学院生化检验教研室
(杜培革，项小丰，付桂莲)，吉林医学院中心医院检验科(李质华) |
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| 摘 要： | 本文采用Sepharose2B层析法去除粗提物中的RNA,用0.7％低融点琼脂糖凝胶回收超螺旋质粒,使质粒的纯化过程费时较少且获得令人满意纯度的超螺旋质粒。
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| 关 键 词： | 质粒 层析 电泳 |
The Extraction, Purification and Identification of Superhelix pGEM - Recombined Plasmid |
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| Du Peige Xiang Xiaofeng Fu Guilian.The Extraction, Purification and Identification of Superhelix pGEM - Recombined Plasmid[J].Journal of Beihua University(Natural Science),1995(3). |
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| Authors: | Du Peige Xiang Xiaofeng Fu Guilian |
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| Abstract: | RNA in raw extract was renoved with Sepharose 2B chromatography, and super-helix plasmid was recovered with 0.7% low melting point agarose gel so that the purification process of plasmid was Less time - consuming and superhelix plasmid with satisfactory purity was obtained . |
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| Keywords: | Piasmid Chromatography Electrophoresis |
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