| 旱柳体外培养与直接分化再生系统的建立 |
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| 引用本文： | 朱丽君,刘关君,田春雨,李洪业,王磊,刘昌财.旱柳体外培养与直接分化再生系统的建立[J].北华大学学报(自然科学版),2010,11(1):63-68. |
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| 作者姓名： | 朱丽君 刘关君 田春雨 李洪业 王磊 刘昌财 |
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| 作者单位： | 东北林业大学,林学院,黑龙江,哈尔滨,150040;吉林市丰满区林业局,吉林,吉林,132013;东北林业大学,林学院,黑龙江,哈尔滨,150040;吉林市林业局,吉林,吉林,132013;吉林市丰满区林业局,吉林,吉林,132013 |
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| 基金项目： | 黑龙江省重点攻关项目 |
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| 摘 要： | 给出了旱柳体外培养的方法:9月份至12月份采集1~2 a生枝条上带腋芽的茎段,经过筛选确定MS+0.5 mg/L 6-BA+0.1 mg/L NAA为最佳诱导培养基,平均每个芽点产生的不定芽数量为3.5个,20 d后苗高为1.8 cm;不定芽在MS+0.1 mg/L 6-BA+0.2 mg/L NAA培养基中继代增殖及生长较好,为适宜的增殖培养基;在形成的不定芽中有些不定芽起源于单株苗的切口基部,因此,初步建立了旱柳的直接分化再生系统,可为旱柳的遗传转化等研究奠定基础.经过壮苗处理的幼苗在MS+0.2 mg/L NAA培养基上的生根率达100%,且生根量多,幼苗生长健壮.生根苗移栽至V(草炭土)V(河沙)为3 1的混合基质中,60 d后成活率为90%左右.
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| 关 键 词： | 旱柳 直接分化再生系统 体外培养 遗传转化 |
Culture in Vitro and Establishment of Regeneration System of Direct Differentiation of Salix matsudana Koidz |
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| ZHU Li-jun,LIU Guan-jun,TIAN Chun-yu,LI Hong-ye,WANG-lei,LIU Chang-cai.Culture in Vitro and Establishment of Regeneration System of Direct Differentiation of Salix matsudana Koidz[J].Journal of Beihua University(Natural Science),2010,11(1):63-68. |
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| Authors: | ZHU Li-jun LIU Guan-jun TIAN Chun-yu LI Hong-ye WANG-lei LIU Chang-cai |
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| Institution: | ZHU Li-jun1,2,LIU Guan-jun1,TIAN Chun-yu3,LI Hong-ye2,WANG-lei1,LIU Chang-cai1(1.College of Forestry,Northeast Forestry University,Harbin 150040,China,2.Fengman District Forestry Bureau in Jilin City,Jilin 132013,3.Jilin City Forestry Bureau,China) |
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| Abstract: | A method is described for culture in vitro of Salix matsudana.On Murashige and Skoog's medium(MS) containing benzylaminopurine(BA) at 0.5mg/L and naphthaleneacetic acid(NAA) at 0.1mg/L3.5-fold shoot multiplication from stem with one axillary bud collected on 1~2 a branch can be achieved,and the adventitious buds were 1.8cm in length after 20d.On MS containing BA at 0.1mg/L and NAA at 0.2mg/L was the best subculture and proliferation medium,adventitious buds produced and some of them directly originated from... |
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| Keywords: | Salix matsudana Koidz regeneration system of direct differentiation culture in vitro genetic transformation |
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