姜黄素对人膀胱癌细胞系T24体外侵袭的作用

目的探讨姜黄素(curcumin)对人膀胱癌细胞系T24体外侵袭能力及其作用机制.方法以不同浓度的姜黄素作用于膀胱癌T24细胞,MTT法检测细胞增殖抑制率,Transwell法实验观察T24侵袭能力的变化,Western blot法检测MMP-2和VEGF蛋白表达的差异.结果姜黄素能有效抑制T24细胞的增殖,并抑制T24细胞的体外侵袭能力,呈时间-剂量依赖性(P0.05).20μmol/L和50μmol/L姜黄素作用细胞24 h后,MMP-2,VEGF蛋白的表达量下降(P0.05).结论姜黄素对T24细胞的增殖和侵袭能力有抑制作用,其机制可能与下调MMP-2和VEGF的蛋白表达有关.

Effect of Curcumin on the Invasion of Bladder Cancer Cell Line T24 in Vitro

Objective To investigate the influence of curcumin on the invasion of bladder cancer cell line T24 in vitro,as well as its probable mechanism. Method T24 cells were treated with different concentrations of curcumin. The effects of the curcumin extract on the proliferation of T24 cells were detected by MTT. Invasive ability was detected by Transwell test, and the expression of MMP-2 and VEGF protein were evaluated by Western blot method. Results The curcumin of different concentrations could significantly inhibit the proliferation and reduce the invasive ability of T24 cells,which was time-dose dependent (P<0. 05). With the intervention of different concentrations of curcumin (20 μmol/L and 50 μmol/L) for 24 h,the expressions of MMP-2 and VEGF protein were decreased ( P<0 . 05 ) . Conclusion Curcumin can inhibit the proliferation and the invasion of T24 cells. The mechanism may be related with the down regulation of the expressions of MMP-2 and VEGF protein.