| 支原体种属特异性PCR引物的设计及对细胞培养物支原体的检测 |
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| 引用本文： | 倪红霞,陈立梅,张雷.支原体种属特异性PCR引物的设计及对细胞培养物支原体的检测[J].北华大学学报(自然科学版),2007,8(6):510-512. |
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| 作者姓名： | 倪红霞 陈立梅 张雷 |
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| 作者单位： | 北华大学,基础医学院,吉林,吉林,132013;军事医学科学院兽医研究所,吉林,长春,130062 |
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| 摘 要： | 目的快速全面检测感染细胞、疫苗等多种生物制品中的支原体.方法从多种支原体的16SrRNA核酸序列中选择了两段高度保守的核酸序列,作为支原体种属特异性引物,对标本进行检测.结果用这对种属特异性引物对已知阳性的50份标本进行检测,检测结果显示全部为阳性.应用于实践,检测了89份疫苗标本,阳性率为31.4%,而用常规培养法检测阳性率为12.3%.结论用该种属特异性引物检测支原体具有检测速度快、检测灵敏度高、特异性强等优点.
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| 关 键 词： | 支原体种属特异性引物 支原体 疫苗 细胞培养物 |
| 文章编号： | 1009-4822（2007）06-0510-03 |
| 收稿时间： | 2007-08-05 |
| 修稿时间： | 2007年8月5日 |
Species-specific PCR for Identification of Common Contaminant Mycoplasma in Cell Culture |
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| NI HONG-Xia,CHEN Li-mei,ZHANG Lei.Species-specific PCR for Identification of Common Contaminant Mycoplasma in Cell Culture[J].Journal of Beihua University(Natural Science),2007,8(6):510-512. |
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| Authors: | NI HONG-Xia CHEN Li-mei ZHANG Lei |
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| Abstract: | Objective To detect mycoplasma species mainly infected in cell cultures and vaccinum.Methods PCR primers were designed according to the published sequences of 16 s rRNA of Mycoplasma in genbank.Mycoplasma species were detected by species-specific PCR.Results To detect 50 samples by using species-specific PCR,the results are all positive.In practice,we detected 89 vaccinum samples by using species-specific PCR method and culture method,31.4% and 12.3% respectively showed the positive.Conclusion The species-specific PCR for mycoplasma could identify mycoplasma with high speed,sensitivity and specification in cell culture. |
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| Keywords: | Species-specific PCR Mycoplasma Vaccinum Cell culture |
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