氟、砷对体外培养大鼠血管内皮细胞的损伤作用

目的观察氟、砷对大鼠血管内皮细胞的损伤作用.方法体外分离培养大鼠胸主动脉血管内皮细胞,浓度按氟化钠(Na F)0,600,900μmol/L与亚砷酸钠(Na As O2)0,0.1,1.0μmol/L配伍,染毒培养时间为48 h.光镜观察细胞生长形态,四甲基偶氮唑盐比色法(MTT法)检测细胞活性,流式细胞仪检测细胞凋亡情况,单细胞凝胶电泳法分析内皮细胞DNA损伤情况.结果非染毒对照组及氟、砷低浓度组细胞生长良好,呈梭形和卵石形,氟、砷单独及联合高浓度组细胞生长状态较差,部分细胞呈圆形,有坏死;氟、砷单独及联合高浓度组细胞活性低于非染毒对照组及氟、砷低浓度组(P0.05);氟、砷单独及联合高浓度组细胞凋亡率高于非染毒对照组及氟、砷低浓度组(P0.05);同样,氟、砷单独及联合高浓度组细胞DNA损伤较非染毒对照组及氟、砷低浓度组严重(P0.05).结论氟、砷单独及联合作用可降低大鼠血管内皮细胞活性,诱导细胞凋亡,引发细胞DNA损伤,并存在明显的剂量-效应关系,提示氟、砷对内皮细胞的生长抑制及DNA损伤作用是血液、循环系统损伤的重要因素.

Damage of Rat Vascular Endothelial Cells Induced by Fluoride and Arsenite in Vitro

Objective To observe the damage effects of fluoride and arsenite on rat vascular endothelial cells. Method Rat aorta endothelial cells were cultured in vitro,and were contaminated with NaF at 0,600 and 900μmol/L and NaAsO2 at 0,0. 1,1. 0 μmol/L compatibility for 48 h. Cell morphology was observed with light microscopy,cell activity was detected by MTT, apoptosis was tested by flow cytometry, and DNA damage in endothelial cells was analyzed through single cell gel electrophoresis. Results The cells of non-exposed control group and the low concentration group grew well,which showed pebble-shaped and spindle-shaped. But the cells, some of what were round with necrosis,appeared poor growth state in the alone or associated high concentrations groups. The cell activity and DNA damage in fluoride and arsenite alone or associated high concentrations groups was lower and more seriously than in non-exposed control group and low concentration group (P<0. 05),and the cells apoptosis showed the opposite result. Conclusion Fluoride and arsenite alone or in combination can reduce the activity of vascular endothelial cells,induce apoptosis and DNA damage,and with an obvious dose-response relationship.