肺癌患者血清P53抗体及肺癌组织P53表达对肺癌诊断价值的研究

目的探讨肺癌患者血清P53抗体水平及肺癌组织P53表达对肺癌诊断的价值.方法采用ELISA法检测肺癌患者血清P53抗体水平,并采用免疫组化法检测肺癌组织P53表达情况.结果肺癌患者血清P53抗体（17.84±8.73）ng/L水平比正常对照组（5.43±1.90）ng/L显著升高（P〈0.001）,肺癌患者血清P53抗体水平与肿瘤大小、淋巴转移、远端转移、TNM分期有关.肺癌患者癌组织P53表达的阳性率为61.5%（168/273）,肺癌患者癌组织P53阳性表达率与肿瘤大小、淋巴转移、远端转移、TNM分期、分化程度、病理类型有关.结论血清P53抗体水平与癌组织中的表达有着良好的平行关系,测定血清P53抗体水平或检查肺癌患者癌组织P53的表达可对肺癌的诊断、分期、治疗和预后提供重要依据.

On Diagnostic Value of Serum P53 Antibody Level and P53 Expression in Patients with Lung Cancer

Objective To investigate the diagnostic value of P53 antibody level and P53 expression in patients with lung cancer. Methods The level of serum P53 antibody and the expression of P53 in lung cancer tissue were detected by enzyme-linked immunosorbent assay （ ELISA） and immunology chemistry,respectively. Results The level of P53 antibody （17. 84±8. 73）ng/L was significantly higher in serum from lung cancer patients than that in serum from normal control group（5. 43±1. 90）ng/L（P〈0. 001）,and was related to the tumor size,lymph node metastasis,distal metastasis and TNM staging. The positive rate of P53 expression was 61. 5%（168/273） in lung cancer tissue, and was related to the size of tumor, lymph node metastasis, distal metastasis, TNM stages, differentiation and pathologic type. Conclusion The level of serum P53 antibody was paralleled to its expression in cancer tissues. The detection of serum P53 antibody level or the tissue expression of P53 in lung cancer tissue can provide an important evidence for the diagnosis,staging,treatment and prognosis of lung cancer.