| 黑果腺肋花楸组培繁殖培养基筛选 |
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| 引用本文： | 陆爽,朱俊义,井桂玲,安岩,陈昌健,孙昊,程广有.黑果腺肋花楸组培繁殖培养基筛选[J].北华大学学报(自然科学版),2018,19(3):395-398. |
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| 作者姓名： | 陆爽 朱俊义 井桂玲 安岩 陈昌健 孙昊 程广有 |
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| 作者单位： | 北华大学林学院,吉林 吉林 132013;通化师范学院生命科学学院,吉林 通化 134002;通化师范学院生命科学学院,吉林 通化,134002;北华大学林学院,吉林 吉林,132013 |
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| 基金项目： | 吉林省科技发展计划项目(20150307010NY) |
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| 摘 要： | 以黑果腺肋花楸茎尖和嫩叶为外植体,在MS培养基中加入不同种类与用量的生长调节剂,筛选黑果腺肋花楸组织培养繁殖培养基.结果表明:黑果腺肋花楸愈伤组织诱导效果较好的培养基为MS+6-BA 3.0 mg/L+NAA 0.5 mg/L;不定芽诱导效果较好的培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L;不定根诱导效果较好的培养基为1/2 MS+IBA 0.05 mg/L;以泥炭土、松针、砂砾(2∶1∶1)为基质时,组培苗移栽成活率较高(成活率96%).
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| 关 键 词： | 黑果腺肋花楸 组织培养 培养基 繁殖 |
Screening of Tissue Culture Medium for Reproduction of Aronia melanocarpa Elliot |
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| Lu Shuang,Zhu Junyi,Jing Guiling,An Yan,Chen Changjian,Sun Hao,Cheng Guangyou.Screening of Tissue Culture Medium for Reproduction of Aronia melanocarpa Elliot[J].Journal of Beihua University(Natural Science),2018,19(3):395-398. |
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| Authors: | Lu Shuang Zhu Junyi Jing Guiling An Yan Chen Changjian Sun Hao Cheng Guangyou |
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| Abstract: | Taking Aronia melanocarpa stem apices and leaves as explants,growth regulators with different kinds and amount cultured in MS culture medium,to screen the culture and propagation of Aronia melanocarpa tissue. The results showed:the better medium of induction effect of Aronia melanocarpa callus was MS+6-BA 3.0 mg/L+NAA 0.5 mg/L;The induction effect of culture medium with good adventitious bud was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L medium;Induction medium of adventitious root was 1/2 MS+IBA 0.05 mg/L.With peat soil,pine needles,gravel (2 : 1 : 1) as the matrix,the transplanting tissue culture seedling has higher survival rate (survival rate 96%). |
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