| 中药材贝母DNA不同提取方法的比较 |
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| 引用本文： | 王淼,谭莹,张丽华.中药材贝母DNA不同提取方法的比较[J].北华大学学报(自然科学版),2011(6):662-665. |
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| 作者姓名： | 王淼 谭莹 张丽华 |
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| 作者单位： | 北华大学医学检验学院;北华大学药学院; |
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| 摘 要： | 目的探讨中药材贝母DNA的不同提取方法,为中药贝母的分子生物学鉴定提供参考.方法利用苯酚法、CTAB法、SDS法从中药贝母中提取DNA,通过紫外分光光度和琼脂糖凝胶电泳对所得DNA样品含量及纯度进行检测.结果利用3种不同的方法提取,药材贝母DNA的A260/A280在1.80±0.23左右.结论利用3种不同DNA提取方...
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| 关 键 词： | 贝母 DNA提取 中药 |
Comparative Study of Different Extraction Methods for the Isolation of DNA from Traditional Chinese Medicine Fritillaria |
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| WANG Miao,TAN Ying,ZHANG Li-hua.Comparative Study of Different Extraction Methods for the Isolation of DNA from Traditional Chinese Medicine Fritillaria[J].Journal of Beihua University(Natural Science),2011(6):662-665. |
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| Authors: | WANG Miao TAN Ying ZHANG Li-hua |
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| Institution: | WANG Miao1,TAN Ying2,ZHANG Li-hua2(1.Medical Inspection College of Beihua University,Jilin 132013,China,2.Pharmaceutical College of Beihua University,China) |
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| Abstract: | Objective To discuss the different extraction methods for the isolation of DNA from traditional Chinese medicine fritillaria and provide the reference for the molecular biology appraisal of the traditional Chinese medicine fritillaria.Method The Phenol,CTAB and SDS methods were used to isolate DNA from the traditional Chinese medicine fritillaria,and ultraviolet spectrophotometry and agarose gel electrophoresis were used to examine DNA content and quality.Results The ratios of A260/A280 for DNA were between... |
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| Keywords: | Fritillaria DNA extraction traditional Chinese medicine |
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