川芎生物碱对大鼠脑缺血-再灌注损伤的保护作用

目的探讨川芎(CHX)生物碱对大鼠脑缺血-再灌注(I/R)损伤的保护作用.方法取Wistar大鼠60只,随机分成6组,每组10只,即假手术组、I/R模型组、川芎生物碱低中高剂量治疗组和阳性药葛根素(GGS)组.采用线栓法建立大鼠大脑中动脉可逆性脑I/R病理损伤模型,用黄嘌呤氧化酶法和硫代巴比妥酸显色法测定脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量.动物苏醒后进行神经功能损伤评分,检测脑组织含水量及容积的变化,同时与假手术组进行比较.结果 I/R实验组与注射生理盐水假手术组比较,脑组织中SOD的活性显著降低,MDA的含量明显增加(P0.01),脑梗死面积和容积明显增大(P0.01),脑组织含水量增多,神经功能损伤较重(P0.05).CHX生物碱治疗组与I/R组比较,脑组织中的SOD的活性显著升高(P0.01),MDA的含量明显降低(P0.05),脑组织含水量减少,脑梗死容积明显缩小(P0.01),神经功能损伤评分差异显著(P0.01).结论 CHX生物碱可减轻大鼠脑I/R引发的脑损伤程度,从而对脑组织具有一定的保护作用.

Protective Effect of Chuanxiong Alkaloid on Cerebral Ischemia Reperfusion Injury in Rats

Objective To explore the protective effects of Chuanxiong ( CHX ) Alkaloids on cerebral ischemia-reperfusion( I/R) injury in rats. Methods A total of 60 wistar rats were purchased and randomly divided into six groups with 10 rats in each grouop, including ischemia-reperfusion ( I/R ) group, sham operated group, the treatment groups and the positive control group. The reversible middle cerebral artery ischemia-reperfusion( I/R) model was made by suture method. Superoxide dismutase( SOD) activity and malondialdehyde( MDA) content of the brain tissue were measured with xanthine oxidase method and thiobarbituric acid method. Neurological injury scores were recorded after the animals regained consciousness,the changes of water content in cerebral tissue was detected,and the results were compared with the sham group. Results Compared with the sham group,in the I/R group,the SOD activity decreased and MDA content increased in brain tissue(P<0. 01),the volume and area of brain infarction significantly increased ( P<0 . 01 ) , the content of brain water increased and neurological seriously damaged(P<0. 05). Compared with I/R group,the SOD activity of CHX Alkaloids treatment groups was significantly higher(P<0. 01),MDA obviously decreased in brain tissue(P<0. 05),water content in brain tissue decreased,the area and volume of infarction significantly reduced(P<0. 01),and neurologic injury score was obviously different(P<0. 01). Conclusion CHX alkaloids can reduce cerebral I/R-induced brain damage,and thus has protective effect on cerebral tissue to some extent.