GRIM-19基因对前列腺癌DU145细胞的促凋亡作用

目的探讨GRIM-19基因对前列腺癌DU145细胞的促凋亡作用,并分析相关机制.方法将构建成功的pGRIM-19重组质粒转染DU145细胞株,应用MTT法检测其对细胞增殖能力的影响,通过流式细胞术检测细胞周期及凋亡率,应用半定量RT-PCR检测GRIM-19及caspase-3 mRNA转录水平.结果流式细胞术结果显示:与对照组比较,pGRIM-19重组质粒明显抑制DU145细胞增殖,并使细胞周期发生改变,多数细胞抑制在G0/G1期,细胞凋亡率增加明显;通过RT-PCR电泳结果证实:pGRIM-19重组质粒明显促进GRIM-19的表达,同时激活caspase-3.结论重组质粒pGRIM-19可明显抑制DU145细胞增殖,并促进其凋亡,其凋亡的发生机制与激活caspase-3有关.

Pro-apoptosis Effect of GRIM-19 Gene on Prostatic Cancer DU145 Cells

Objective To explore the pro-apoptosis effect of GRIM-19 gene on prostatic cancer DU145 cells and analyze the related mechanisms.Method The constructed recombinant plasmid pGRIM-19 was transfected into prostatic carcinoma DU145 cells.The growth inhibitory rate was determined by MTT,cell cycle distribution and apoptosis rate were determined with flow cytometry and GRIM-19 and caspase-3 mRNA expression levels were measured by using RT-PCR assay.Results Compared with control group,pGRIM-19 could inhibit DU145 cells proliferation obviously.The cell cycle changed,most cells remained in G0/G1 phase with obvious apoptotic rate by flow cytometry;pGRIM-19 could promote GRIM-19 expression and activate caspase-3 through RT-PCR detection.Conclusion The recombinant plasmid pGRIM-19 could significantly inhibit DU145 cells proliferation and promote their apoptosis.The mechanism was related to high caspase-3 level.