驱蚊草组织培养及其再生体系的建立与优化

通过对驱蚊草离体叶片和茎段的培养及植株再生的研究,成功地建立了驱蚊草组织培养快繁技术体系.用0.1%升汞对叶片、叶柄和茎段进行消毒,最佳消毒时间分别为6.5、6.0、7.0 m in;叶片和茎段不定芽诱导的最适培养基为M S BA(1.0 m g/L) NAA(1.0 m g/L),叶柄为M S BA(0.5 m g/L) NAA(0.5 m g/L);不定芽的最适增殖培养基为M S BA(0.75 m g/L) NAA(0.6 m g/L) GA3(0.2 m g/L),增殖倍数为6.1;最适生根培养基为1/2 M S培养基,生根率92%,平均每株生根数为10条.

The Establishment and Optimization of the Regeneration System of Mozzie buster in Vitro

By studying the culture of the detached leaves and stems and the regeneration of Mozzie buster plantlets,the experimental-system of rapid propagation of Mozzie buster in vitro have been successfully established.With 0.1% HgCl_2,the optimum sterilization times for leaves,petioles and stems are 6.5,6.0 ,7.0 min respectively.The most suitable medium for the initiation of adventitious buds from leaves and stems is MS medium supplemented with 1.0 mg/L BA and 1.0 mg/L NAA,and that from petioles is MS medium supplemented with 0.5 mg/L BA and 0.5 mg/L NAA.The best medium for the propagation of adventitious buds is MS medium supplemented with 0.75 mg/L BA,0.6 mg/L NAA and 0.2 mg/L GA_3. The ratio of buds propagation is 6.1,1/2 MS medium is most suitable for the rooting of shoots.The rate of rooted shoots is 92% and the average number of roots per shoot is 10.