花生白藜芦醇合酶基因转化单子叶植物表达载体的构建

构建了花生白藜芦醇合酶基因(RS)转化单子叶植物的表达载体,该表达载体含有ubi 启动子和内含子,能启动该基因在单子叶植物中高效地表达.通过PCR反应扩增出目的片段,连接到克隆载体Pubi35s上,切下含ubi和RS约3 000 bp的片段连接到植物表达载体pCAMBIA-1 380上.经PCR和酶切检测,结果与预期相同,经测序确定插入片段读码框正确.该表达载体可用于单子叶植物高效的表达.

Construction of the Monocotyledon Expression Vector for the Transforming of Resveratrol Synthase Gene

A monocotyledon expression vector was constructed for transforming Resveratrol Synthase(RS) gene.This vector contains ubi promoter and intron which can drive high expression of the gene in monocotyledon.PCR was used to amplify the RS gene.A new cloning vector was then constructed by inserting the amplified RS gene into Pubi35s.The monocotyledon expression vector was constructed by inserting the ubi promoter and RS gene(about 3 000 bp) into pCAMBIA-1 380,which cutted from the new cloning vector above.PCR and restriction enzyme digesting analysis showed that the inserting DNA fragment was the size of expecting.Furthermore,the sequencing showed that the insert DNA fragment was not out of frame.The expression vector can be used for the high expression in monocotyledon.