| 裸盖菇L-001菌株液体培养及其产毒素初探 |
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| 引用本文： | 毛宁,丁珊珊,余宗钟.裸盖菇L-001菌株液体培养及其产毒素初探[J].福建师范大学学报(自然科学版),2009,25(6). |
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| 作者姓名： | 毛宁 丁珊珊 余宗钟 |
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| 作者单位： | 福建师范大学生命科学学院,教育部工业微生物工程中心,福建,福州,350108 |
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| 基金项目： | 福建省自然科学基金资助项目，福建师范大学大学生课外科技项目 |
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| 摘 要： | 用5种不同碳源、氮源进行液体发酵培养裸盖菇L-001菌株,探讨最适其产毒素的碳、氮因子,研究其生物量与毒素的关系.结果发现:裸盖菇菌丝体生物量与其毒素产量呈现正相关,最适产毒素的碳源为马铃薯淀粉,氮源为黄豆粉.裸盖菇菌丝体破壁液和发酵液对绿豆芽RNA聚合酶Ⅱ均具有阻断抑制作用,小鼠实验也表明两者均使小鼠出现神经中毒现象.
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| 关 键 词： | 裸盖菇 液体发酵 毒素 抑芽法 |
Exploratiopn of the Psilocybe-L-001's Liquid Culture and Toxin Production |
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| MAO Ning,DING Shan-shan,YU Zong-zhong.Exploratiopn of the Psilocybe-L-001's Liquid Culture and Toxin Production[J].Journal of Fujian Teachers University(Natural Science),2009,25(6). |
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| Authors: | MAO Ning DING Shan-shan YU Zong-zhong |
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| Institution: | MAO Ning,DING Shan-shan,YU Zong-zhong(College of Life Sciences,Fujian Normal University,The Ministry of Education's Engineering Center of Industrial Microbiology,Fuzhou 350108,China) |
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| Abstract: | In the basis of the study of biological characteristics of strain L-001,used 5 different carbon source and nitrogen to make liquid culture. The results show: Biomass and the amount of toxin have positive correlation, potato starch is the best carbon source and soybean meal is the best nitrogen. The mycelium broth and broken extract have an inhibition effect of mung beans' RNA Polymerase I . Mouse test also shows that the mycelium broth and broken extract can make mice neurotoxic. |
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| Keywords: | Psilocybe liquid culture toxin Bud-inhibited assay |
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