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壮药咳嗽草中总黄酮提取工艺研究
引用本文:韦波,杨瑞云,刘静,薛佳津,苏小莹,姚茜芸.壮药咳嗽草中总黄酮提取工艺研究[J].广西师范大学学报(自然科学版),2012,30(2):83-87.
作者姓名:韦波  杨瑞云  刘静  薛佳津  苏小莹  姚茜芸
作者单位:广西师范大学化学化工学院,药用资源化学与药物分子工程国家重点实验室培育基地,广西桂林541004
基金项目:973计划前期研究专项资助项目,广西科技厅应用基础研究资助项目,药用资源化学与药物分子工程教育部重点实验室主任基金,广西自然科学基金创新团队项目,广西师范大学创新性实验计划项目
摘    要:利用单因素试验和正交试验相结合的方法,考察溶剂浓度、液料比、提取时间、提取次数对咳嗽草中总黄酮提取效果的影响。结果表明,咳嗽草中总黄酮最佳提取工艺为80%甲醇、液料比10∶1、回流提取2h、提取4次,所得总黄酮含量达到1.30%。该工艺简单易行,是提取咳嗽草中总黄酮的有效途径。

关 键 词:咳嗽草  总黄酮  提取工艺  正交试验

Extraction Techniques of Total Flavonoids in Flemingia Strobilifera
WEI Bo , YANG Rui-yun , LIU Jing , XUE Jia-jin , SU Xiao-ying , YAO Qian-yun.Extraction Techniques of Total Flavonoids in Flemingia Strobilifera[J].Journal of Guangxi Normal University(Natural Science Edition),2012,30(2):83-87.
Authors:WEI Bo  YANG Rui-yun  LIU Jing  XUE Jia-jin  SU Xiao-ying  YAO Qian-yun
Institution:(State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources,College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin Guangxi 541004,China)
Abstract:In order to optimize the extraction techniques of total flavonoids in Flemingia strobilifera,effects of concentration of solvent,the radio liquid to material,time length of extraction,and frequency of extraction on F.strobilifera total flavonoids yield were studied by single factor experiments and orthogonal experiments.The results showed that the optimum extraction techniques were extracting in 80% methanol with the ratio of liquid to material at 10∶1 for 4 times,with 2 hours for each time.The content of total flavonoids was 1.30% in extract.The optimized techniques are simple and feasible,which is an effective way to extract total flavonoids from F.strobilifera.
Keywords:Flemingia strobilifera  total flavonoids  extraction techniques  orthogonal test
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