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不同培养条件对益母草悬浮培养细胞生长的影响
引用本文:李学栋,龚真才,马勇,何平,杨军.不同培养条件对益母草悬浮培养细胞生长的影响[J].西华师范大学学报(哲学社会科学版),2008,29(2):136-140.
作者姓名:李学栋  龚真才  马勇  何平  杨军
作者单位:[1]西华师范大学生命科学学院,四川南充637002 [2]四川省大竹中学,四川大竹635100 [3]内江第六中学,四川内江641000
基金项目:四川省重点学科建设项目(SZD0420)
摘    要:测定了不同的基本培养基,不同的激素水平,不同的糖含量以及不同的初始接入量对益母草悬浮培养细胞生长的影响.结果发现MS培养基最益于悬浮培养细胞的生长,3%的蔗糖水平细胞的生物量增长率最大.在初始接入量为6g/L时细胞增殖最快,在低接入量的情况下细胞停止生长.在6-BA无论与2,4-D组合还是与NAA组合,均在细胞分裂素2mg/L和生长素0.5mg/L时悬浮培养细胞的增殖率达到最大.研究结果为细胞培养生产益母草生物碱提供参考依据.

关 键 词:益母草  悬浮培养  培养条件

Effect of Different Culture Conditions on Cell Growth in Suspension Culture of L.heterophyllus
LI Xue-dong,GONG Zhen-cai,MA Yong,He Ping,YANG Jun.Effect of Different Culture Conditions on Cell Growth in Suspension Culture of L.heterophyllus[J].Journal of China West Normal University:Natural Science Edition,2008,29(2):136-140.
Authors:LI Xue-dong  GONG Zhen-cai    MA Yong  He Ping  YANG Jun
Institution:LI Xue-dong1,GONG Zhen-cai1,2,MA Yong1,He Ping3,YANG Jun1(1.College of Life Sciences,China West Normal University,Nanchong 637002,2.Dazhu Middle School,Dazhu 635100,China,3.Neijing No.6 Middle School,Neijiang 641000,China)
Abstract:Callus of Leonurus heterophyllus Sw.from solid medium were cultured in liquid medium,and the types of basal medium,concentration of plant growth regulators,content of sucrose in medium,and quantity of original incubated callus were respectively tested on the increase of callus proliferation.It was found that MS medium was most suitable for suspended cell growth,and the biomass of the cells increased rapidly in medium with 3% sucrose.6g/L original incubated callus showed the most promptional effect on callus...
Keywords:Leonurus heterophyllus Sw    suspension culture  culture conditions  
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