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百合中秋水仙碱的分离及结构表征
引用本文:何纯莲,任凤莲,吴泓毅,陈蓉.百合中秋水仙碱的分离及结构表征[J].中南大学学报(自然科学版),2006,37(2):286-289.
作者姓名:何纯莲  任凤莲  吴泓毅  陈蓉
作者单位:1. 中南大学,化学化工学院,湖南,长沙,410083;湖南师范大学,医学院,湖南,长沙,410006
2. 中南大学,化学化工学院,湖南,长沙,410083
3. 湖南师范大学,医学院,湖南,长沙,410006
基金项目:湖南省教育厅自然科学基金
摘    要:采用超临界二氧化碳流体萃取百合中秋水仙碱, 用高效液相色谱法对萃取物中秋水仙碱的含量进行测定, 用重结晶、薄层色谱法对萃取物中秋水仙碱进行分离纯化, 纯化后的目标物用核磁共振和红外光谱进行表征. 研究结果表明: 采用高效液相色谱法测得萃取物中秋水仙碱的质量分数为6.4%, 纯化后分离收率为88%, 核磁共振和红外光谱表征的结果与标准物的图谱一致, 可见超临界流体萃取-测定-分离百合中秋水仙碱的方法具有较好的选择性.

关 键 词:百合  秋水仙碱  超临界流体  萃取  分离
文章编号:1672-7207(2006)02-0286-04
收稿时间:2005-08-12
修稿时间:2005年8月12日

Separation and identification of colchicine from lily
HE Chun-lian,REN Feng-lian,WU Hong-yi,CHEN Rong.Separation and identification of colchicine from lily[J].Journal of Central South University:Science and Technology,2006,37(2):286-289.
Authors:HE Chun-lian  REN Feng-lian  WU Hong-yi  CHEN Rong
Institution:1, School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China 2. College of Medicine, Hunan Normal University, Changsha 410006, China
Abstract:The colchicine content of lily in supercritical carbon dioxide fluid extraction was deter mined by high performance liquid chromatography. Pure colchicine was separated from the extracts through thin layer chromatography and was identified by using spectral techniques of nuclear magnetic resonance and infrared spectrum. The results show that colchicine content is increased to 6.4% in extract. The recovery of colchicine is 88%, and spectral diagram of colchicine from lily is the same as that of colchicine standard sample. The method of extraction-detectionseparation colchicine from lily is applicable and it has a preferable selectivity.
Keywords:lily  colchicine  supercritical fluid  extraction  separation
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