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制备型高效液相色谱法分离纯化绿原酸
引用本文:彭密军,周春山,钟世安,刘斌.制备型高效液相色谱法分离纯化绿原酸[J].中南大学学报(自然科学版),2004,35(3):408-412.
作者姓名:彭密军  周春山  钟世安  刘斌
作者单位:1. 中南大学,化学化工学院,湖南长沙,410083;吉首大学,湖南省林产化工重点实验室,湖南张家界,427000
2. 中南大学,化学化工学院,湖南长沙,410083
基金项目:湖南省科技厅攻关项目,科技部农业科技成果转化基金,湖南省教育厅科研项目
摘    要:采用制备高效液相色谱技术,从杜仲叶提取物中分离纯化得到高纯活性成分绿原酸.对制备色谱的洗脱方式、洗脱剂组成、浓度、洗脱速度等参数进行优化,提出并应用了流动相速度梯度洗脱方法.最佳操作条件为:采用流速梯度进行洗脱,流速先为28 mL/min,然后为45 mL/min;流动相为15%的有机酸B和 0.1%的有机酸E(体积分数);测定波长为254 nm;进样体积为10 mL;进样量为2500 mg.通过该工艺分离纯化,制备回收率为83.3%,相对标准偏差为3.1%,绿原酸的纯度达98.61%,同时可得到杜仲叶黄酮、桃叶珊瑚甙等副产品.

关 键 词:绿原酸  高效液相制备色谱  纯化  流速梯度洗脱
文章编号:1672-7207(2004)03-0408-05
修稿时间:2003年7月15日

Separation and purification of chlorogenic acid by preparative high performance liquid chromatography
PENG Mi-jun.Separation and purification of chlorogenic acid by preparative high performance liquid chromatography[J].Journal of Central South University:Science and Technology,2004,35(3):408-412.
Authors:PENG Mi-jun
Institution:PENG Mi-jun~
Abstract:Preparative high performance liquid chromatography (PHPLC) technique was used to separate and purify high purity of chlorogenic acid from Eucommia ulmoides Oliv. The elution mode, composition of the mobile phase, content of the organic acid and the flow-rate of the mobile phase were optimized and thus a new flow rate gradient was proposed. The optimum operation parameters were: the flow rate gradient of 28 mL/min first and 45 mL/min later, the mobile phase of 15% alcohol B added with 0.1% organic acid E(volume fraction), the determination wavelength of 254 nm, the injecting volume of 10 mL and the injecting mass of 2 500 mg. The average recovery rate was 83.8% and relative standard deviation(RSD) was 3.1%. High purity product of chlorogenic acid ((98.61%)) was obtained and some by-products such as aucubin and flavone were also obtained.
Keywords:chlorogenic acid  preparative high performance liquid chromatography  purification  flow rate gradient
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