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| 中等嗜热菌浸出黄铜矿的温度影响规律及种群组成分析 | |
| 引用本文： | 温建康,徐金光,陈勃伟,武彪,姚国成,刘美林,王淀佐.中等嗜热菌浸出黄铜矿的温度影响规律及种群组成分析[J].中南大学学报(自然科学版),2012,43(1):1-7. |
| 作者姓名： | 温建康 徐金光 陈勃伟 武彪 姚国成 刘美林 王淀佐 |
| 作者单位： | 1. 北京有色金属研究总院生物冶金国家工程实验室,北京,100088 2. 中国瑞林工程技术有限公司,江西南昌,330002 3. 北京有色金属研究总院生物冶金国家工程实验室,北京,100088;北京科技大学土木与环境工程学院,北京,100083 4. 北京有色金属研究总院生物冶金国家工程实验室,北京,100088;中国工程院,北京,100088 |
| 基金项目： | 国家高技术研究发展计划(“863”计划)项目(2007AA060900);国家自然科学基金重点资助项目(50934002);国家科技支撑计划项目(2006BAB02A08) |
| 摘 要： | 在不同温度条件下进行浸出实验.研究结果表明:中等嗜热菌在12～55℃时可保持浸矿活性,其最适生长温度为45～50℃,温度达到60℃时初始阶段还保持一定活性,但几天后菌浓度逐渐降低,种群比例发生较大变化；克隆文库分析表明:45℃和60℃条件下浸矿细菌主要为Leptospirillum ferriphilum,Acidithiobacillus caldus,Sulfobacillus thermotolerans和Acidosphaera rubrifaciens,其中Leptospirillum ferriphilum菌所占比例较大,是浸矿过程中的优势菌种.60℃条件下浸出过程中还存在古生菌Ferroplasma cupricumulans,古生菌可以利用其他浸矿菌产生的有机物为能源生长.它们之间对浸出所起的作用是相辅相成的,可共同促进黄铜矿的溶解. |
| 关 键 词： | 细菌浸出 黄铜矿 中等嗜热菌 克隆文库 |
Temperature influence on chalcopyrite bioleaching by moderate thermophile bacteria and microbial population analysis |
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| WEN Jian-kang , XU Jin-guang , CHEN Bo-wei , WU Biao , YAO Guo-cheng , LIU Mei-lin , WANG Dian-zuo.Temperature influence on chalcopyrite bioleaching by moderate thermophile bacteria and microbial population analysis[J].Journal of Central South University:Science and Technology,2012,43(1):1-7. | |
| Authors: | WEN Jian-kang XU Jin-guang CHEN Bo-wei WU Biao YAO Guo-cheng LIU Mei-lin WANG Dian-zuo |
| Institution: | 1,4 (1.National Engineering Laboratory of Biohydrometallurgy,General Research Institute for Nonferrous Metals, Beijing 100088,China; 2.China Nerin Engineering Co.,Ltd.,Nanchang 330002,China; 3.School of Civil and Environmental Engineering,University of Science & Technology Beijing,Beijing 100083,China; 4.Chinese Academy of Engineering,Beijing 100088,China) |
| Abstract: | Bioleaching of chalcopyrite with moderate thermophilic bacteria was conducted at different temperatures.The results show that the moderate thermophiles can present bioleaching ability at 12-55 ℃ during leaching process.The optimum growth temperature is 45-50℃.When the temperature reaches 60 ℃,moderate thermophiles can maintain activity in the initial stage,but after a few days the bacteria concentration reduces gradually.16S rDNA clone library reveals that the composition of moderate thermophiles at 45 ℃ and 60 ℃ are mainly Leptospirillum ferriphilum,Acidithiobacillus caldus,Sulfobacillus thermotolerans and Acidosphaera rubrifaciens.Leptospirillum ferriphilum is the dominant bacteria at the both temperatures,and archaea Ferroplasma cupricumulans are found at 60 ℃,which can use organic substances produced by other bacteria as energy source.It is suggested that these bacteria can supplement each other during bioleaching,thus facilitating the dissolution of chalcopyrite. |
| Keywords: | bioleaching chalcopyrite moderate thermophile clone library |
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