| 甜菜抗根腐病基因ISSR分子标记的初步研究 |
| |
| 引用本文： | 吴旭红.甜菜抗根腐病基因ISSR分子标记的初步研究[J].齐齐哈尔大学学报(自然科学版),2005,21(2):30-32. |
| |
| 作者姓名： | 吴旭红 |
| |
| 作者单位： | 齐齐哈尔大学生命科学与工程学院,黑龙江,齐齐哈尔,161006 |
| |
| 基金项目： | 黑龙江省教育厅项目。项目编号:10551332 |
| |
| 摘 要： | 以感病×抗病的甜菜种间杂交组合KWS9419×ZD204的F1代17个单株及ZD自交一代16个单株为试材,采用BSA法和ISSR技术,通过对155个随机引物的筛选,获得了一个与甜菜抗根腐病基因连锁的ISSR标记OPP0760,可以用作抗根腐病基因的分子辅助选择的依据。
|
| 关 键 词： | 甜菜 抗根腐病基因 ISSR分子标记法 病害 核苷酸 |
| 文章编号： | 1007-984X(2005)02-0030-03 |
| 修稿时间： | 2005年1月14日 |
Identification of molecular genetic markers tightly linked to root rot resistant genes in sugar beet |
| |
| WU xu-hong.Identification of molecular genetic markers tightly linked to root rot resistant genes in sugar beet[J].Journal of Qiqihar University(Natural Science Edition),2005,21(2):30-32. |
| |
| Authors: | WU xu-hong |
| |
| Abstract: | Bulk segregant analysis (BSA), randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) methords were used to tag the root rot-resistant genes of Sugar beet with 17 individuals of F1 progeny between ZD204,root rot- resistant and Ugni Blanc , root rot -sensitive and 16 individuals self-pollinated of ZD204. Among 155 random primers gave distinct band Patterns , One RAPD marker OPP084760 was tightly linked to a major gene resistant to root rot. Marker OPP084760 was cloned and sequenced. It could be used for the identification of hybrid resistant to root rot |
| |
| Keywords: | sugar beet root rot resistance gene ISSR marker |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
