Evolutionary relationship and species separation of four morphologically similar stichotrichous ciliates (Protozoa, Ciliophora) |
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作者单位: | Zhenzhen Yi,Zigui Chen,Weibo Song(Laboratory of Protozoology,Ocean University of China,Qingdao 266003,China);John Clamp(Department of Biology,North Carolina Central University,Durham,NC 27707,USA);Khaled A.S.A1-Rasheid,Abdulaziz A.Al-Khedhairy(Zoology Department,King Saud University,Riyadh 11451,Saudi Arabia)
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基金项目: | 国家自然科学基金,the Center of Excellence in Biodiversity Research and the DNA Research Chair Program |
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摘 要: |
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关 键 词: | 纤毛虫 分离 原生动物 类似 进化关系 物种 形态 系统发育分析 |
收稿时间: | 11 April 2008 |
修稿时间: | 4 May 2008 |
Evolutionary relationship and species separation of four morphologically similar stichotrichous ciliates (Protozoa, Ciliophora) |
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Authors: | Zhenzhen Yi John Clamp Khaled ASA-Rasheid Abdulaziz AAl-Khedhairy Zigui Chen Weibo Song |
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Institution: | 1. Laboratory of Protozoology,Ocean University of China,Qingdao 266003,China 2. Department of Biology,North Carolina Central University,Durham,NC 27707,USA 3. Zoology Department,King Saud University,Riyadh 11451,Saudi Arabia |
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Abstract: | Phylogenetic and taxonomic studies on ciliate protists using molecular approaches have been demonstrated to be very reliable to form strong conclusions and results. In the present work, species separation of some morphologically similar stichotrichous ciliates, two species of Pseudokeronopsis and two species of Apokeronopsis, was reexamined using amplified ribosomal DNA restriction analysis (PCRRFLP). Five of 10 restriction enzymes revealed species-specific polymorphic patterns, of which four similar stichotrichs could be significantly separated and identified. Among them, EcoR I offered almost no significantly different restriction fragment patterns, but the four species could be separated from one another and identified with Hae III. Distinctly different restriction digestion haplotypes and similarity indices separated the species, and were used to construct a phylogeny. Phylogenies based on ITS2 nucleotide sequences and ITS2 secondary structures supported the separation of Pseudokeronopsis and Apokeronopsis using RFLP analysis, although three Pseudokeronopsis carnea populations did not cluster together. In addition, phylogenetic analyses using multiple algorithms confirmed that these two genera formed two distinct groups within the urostylids. |
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Keywords: | Ciliophora Pseudokeronopsis Apokeronopsis PCR-RFLP ITS2 secondary structures |
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