Hydrogen production by mixed culture of several facultative bacteria and anaerobic bacteria

The characteristic of hydrogen production by facultative anaerobic bacteria, obligate anaerobic bacteria and their mixed culture was studied by the batch culture method. The results showed that, due to the synergistic effect between facultative bacteria and anaerobic bacteria, the ability of hydrogen production in the mixed culture was much better than that in the pure culture. Especially, the culture Scheme No.7 mixed up with three strains ( Bacterium.E: Bacterium.B: Bacterium.P = 1:1:1) not only had the best hydrogen production capacity (1.885 mol H2/mol glucose) and maximum average hydrogen production rate (212.2mL/(L·h)), but also had stable hydrogen production under continuous culture conditions, which was 1.968 mol H2/mol glucose.