| Genetic mapping of telomere-associated sequences in soybean ( Glycine max ) |
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| 作者姓名： | 刘峰 张德水 张劲松 陈受宜 |
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| 作者单位： | Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China,Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China,Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China,Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China |
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| 基金项目： | Project supported by NKBRSF, China, (No. 1998011703) and the National 863 High-Tech Program, China (Grant No.863-101-02-02-03). |
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| 摘 要： | Two telomere-associated sequences (TAS), named STAS8 and STAS10, were cloned from soybean genomic DNA using polymerase chain reaction (PCR) amplification. Southern analysis showed that they were sequences with moderate copy number in soybean genome. Sequence analysis demonstrated that STAS10 had tandemly arrayed con sensus sequences of TTTAGGG and TIAGGG . The mapping of these two TAS was performed with a population of F8 re combinant inbred line using restriction fragment length polymorphisms(RFLP). Seven out of nine polymorphic fragments were mapped to the most distal position of five linkage groups, Dla, F, G2, H and Q of soybean, and the other two loci were closely linked and mapped to two interstitial positions within linkage group D1a. The mapping of TAS in soybean is essential for completeness of a molecular genetic map of soybean.
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| 关 键 词： | soybean (Glycine max) telomere-associated sequence (TAS) genetic mapping |
Genetic mapping of telomere-associated sequences in soybean ( Glycine max ) |
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| LIU Feng,ZHANG Deshui,ZHANG Jinsong,CHEN Shouyi.Genetic mapping of telomere-associated sequences in soybean ( Glycine max )[J].Progress in Natural Science,2001,11(5):351-357. |
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| Authors: | LIU Feng ZHANG Deshui ZHANG Jinsong CHEN Shouyi |
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| Institution: | Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Two telomere-associated sequences (TAS), named STAS8 and STAS10, were cloned from soybean genomic DNA using polymerase chain reaction (PCR) amplification. Southern analysis showed that they were sequences with moderate copy number in soybean genome. Sequence analysis demonstrated that STAS10 had tandemly arrayed con sensus sequences of TTTAGGG and TIAGGG . The mapping of these two TAS was performed with a population of F8 re combinant inbred line using restriction fragment length polymorphisms(RFLP). Seven out of nine polymorphic fragments were mapped to the most distal position of five linkage groups, Dla, F, G2, H and Q of soybean, and the other two loci were closely linked and mapped to two interstitial positions within linkage group D1a. The mapping of TAS in soybean is essential for completeness of a molecular genetic map of soybean. |
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| Keywords: | soybean (Glycine max) telomere-associated sequence (TAS) genetic mapping |
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