External pH regulates the inward K+ channels in Brassica pollen protoplasts |
| |
Authors: | FAN Liumin WU Weihua |
| |
Institution: | Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China,Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China |
| |
Abstract: | Patch-clamp whole-cell recording techniques were used to investigate the regulation of the inward K* channels by external pH in Brassica pollen protoplasts. The inward K+ currents, conductance and voltage-independent maximum conductance were not significantly affected when the external pH was changed from 5.8 to 4.5. However, compared with the results obtained at pH 5.8, the inward K+ currents and conductance were significantly inhibited at pH 8 .5 . The activation kinetics of the inward K * channels was also significantly regulated by external pH. The channel activation time was shorter at the lower external pH than that at higher pH. Since external pH is a critical factor to regulate pollen germination, the results suggest that external pH regulation of the inward K+ channels may be one of potential physiological mechanisms for the regulation of pollen germination. |
| |
Keywords: | Brassica pollen protoplast pollen germination K* channel pH |
|
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 |
|